Dystroglycan depletion inhibits the functions of differentiated HL-60 cells

Alma Delia Martínez-Zárate; Ivette Martínez-Vieyra; Lea Alonso-Rangel; Bulmaro Cisneros; Steve J. Winder; Doris Cerecedo

doi:10.1016/j.bbrc.2014.04.110

Dystroglycan depletion inhibits the functions of differentiated HL-60 cells

Alma Delia Martínez-Zárate, Ivette Martínez-Vieyra, Lea Alonso-Rangel, Bulmaro Cisneros, Steve J. Winder, Doris Cerecedo

Escuela Nacional de Medicina y Homeopatía (ENMH)

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Dystroglycan has recently been characterized in blood tissue cells, as part of the dystrophin glycoprotein complex but to date nothing is known of its role in the differentiation process of neutrophils. We have investigated the role of dystroglycan in the human promyelocytic leukemic cell line HL-60 differentiated to neutrophils. Depletion of dystroglycan by RNAi resulted in altered morphology and reduced properties of differentiated HL-60 cells, including chemotaxis, respiratory burst, phagocytic activities and expression of markers of differentiation. These findings strongly implicate dystroglycan as a key membrane adhesion protein involved in the differentiation process in HL-60 cells.

Original language	English
Pages (from-to)	274-280
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	448
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2014.04.110
State	Published - 6 Jun 2014

Keywords

Differentiation
Dystroglycan
HL-60 cells
Migration
Phagocytosis
Respiratory burst activity

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10.1016/j.bbrc.2014.04.110

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title = "Dystroglycan depletion inhibits the functions of differentiated HL-60 cells",

abstract = "Dystroglycan has recently been characterized in blood tissue cells, as part of the dystrophin glycoprotein complex but to date nothing is known of its role in the differentiation process of neutrophils. We have investigated the role of dystroglycan in the human promyelocytic leukemic cell line HL-60 differentiated to neutrophils. Depletion of dystroglycan by RNAi resulted in altered morphology and reduced properties of differentiated HL-60 cells, including chemotaxis, respiratory burst, phagocytic activities and expression of markers of differentiation. These findings strongly implicate dystroglycan as a key membrane adhesion protein involved in the differentiation process in HL-60 cells.",

keywords = "Differentiation, Dystroglycan, HL-60 cells, Migration, Phagocytosis, Respiratory burst activity",

author = "Mart{\'i}nez-Z{\'a}rate, {Alma Delia} and Ivette Mart{\'i}nez-Vieyra and Lea Alonso-Rangel and Bulmaro Cisneros and Winder, {Steve J.} and Doris Cerecedo",

note = "Funding Information: This work was supported by CONACYT -M{\'e}xico, grant no. 161781 . We thank the Unidad de Microscop{\'i}a Confocal (Centro de Investigaci{\'o}n y Estudios Avanzados del IPN) for technical assistance.",

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doi = "10.1016/j.bbrc.2014.04.110",

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T1 - Dystroglycan depletion inhibits the functions of differentiated HL-60 cells

AU - Martínez-Zárate, Alma Delia

AU - Martínez-Vieyra, Ivette

AU - Alonso-Rangel, Lea

AU - Cisneros, Bulmaro

AU - Winder, Steve J.

AU - Cerecedo, Doris

N1 - Funding Information: This work was supported by CONACYT -México, grant no. 161781 . We thank the Unidad de Microscopía Confocal (Centro de Investigación y Estudios Avanzados del IPN) for technical assistance.

PY - 2014/6/6

Y1 - 2014/6/6

N2 - Dystroglycan has recently been characterized in blood tissue cells, as part of the dystrophin glycoprotein complex but to date nothing is known of its role in the differentiation process of neutrophils. We have investigated the role of dystroglycan in the human promyelocytic leukemic cell line HL-60 differentiated to neutrophils. Depletion of dystroglycan by RNAi resulted in altered morphology and reduced properties of differentiated HL-60 cells, including chemotaxis, respiratory burst, phagocytic activities and expression of markers of differentiation. These findings strongly implicate dystroglycan as a key membrane adhesion protein involved in the differentiation process in HL-60 cells.

AB - Dystroglycan has recently been characterized in blood tissue cells, as part of the dystrophin glycoprotein complex but to date nothing is known of its role in the differentiation process of neutrophils. We have investigated the role of dystroglycan in the human promyelocytic leukemic cell line HL-60 differentiated to neutrophils. Depletion of dystroglycan by RNAi resulted in altered morphology and reduced properties of differentiated HL-60 cells, including chemotaxis, respiratory burst, phagocytic activities and expression of markers of differentiation. These findings strongly implicate dystroglycan as a key membrane adhesion protein involved in the differentiation process in HL-60 cells.

KW - Differentiation

KW - Dystroglycan

KW - HL-60 cells

KW - Migration

KW - Phagocytosis

KW - Respiratory burst activity

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U2 - 10.1016/j.bbrc.2014.04.110

DO - 10.1016/j.bbrc.2014.04.110

M3 - Artículo

C2 - 24792180

SN - 0006-291X

VL - 448

SP - 274

EP - 280

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Dystroglycan depletion inhibits the functions of differentiated HL-60 cells

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Keywords

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