Development of DNA biosensors for single nucleotide polymorphisms

N. M. Pérez-Vielma; G. D. Ibarra-García; M. Gómez-López; M. A. Martínez Godínez; H. González Martínez; C. A. González Díaz; B. E. Del-Río-Navarro; V. F. Martínez-Silva; A. Martínez-Rivas; A. V. Vargas Varval; A. Miliar-García

doi:10.1007/978-3-319-13117-7_15

Development of DNA biosensors for single nucleotide polymorphisms

N. M. Pérez-Vielma, G. D. Ibarra-García, M. Gómez-López, M. A. Martínez Godínez, H. González Martínez, C. A. González Díaz, B. E. Del-Río-Navarro, V. F. Martínez-Silva, A. Martínez-Rivas, A. V. Vargas Varval, A. Miliar-García

Producción científica: Capítulo del libro/informe/acta de congreso › Contribución a la conferencia › revisión exhaustiva

Resumen

The development of a DNA biosensor for the detection of any single nucleotide polymorphism (SNP) implemented on the basis of a quartz crystal resonator as transducer is presented. The methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C SNPs were chosen to perform a proof of concept. We designed and proved a specific surface chemistry on gold in order to use human DNA with no chemical modification as probe. The immobilization of the human DNA via the proposed surface chemistry was successfully characterized with an Atomic Force Microscope (AFM). The DNA biosensor proposed here results in a specific and rapid manner to detect the evaluated SNP.

Idioma original	Inglés
Título de la publicación alojada	VI Latin American Congress on Biomedical Engineering, CLAIB 2014
Editores	Ariel Braidot, Alejandro Hadad
Editorial	Springer Verlag
Páginas	55-58
Número de páginas	4
ISBN (versión digital)	9783319131160
DOI	https://doi.org/10.1007/978-3-319-13117-7_15
Estado	Publicada - 2015
Evento	6th Latin American Congress on Biomedical Engineering, CLAIB 2014 - Paraná, Argentina Duración: 29 oct. 2014 → 31 oct. 2014

Serie de la publicación

Nombre	IFMBE Proceedings
Volumen	49
ISSN (versión impresa)	1680-0737

Conferencia

Conferencia	6th Latin American Congress on Biomedical Engineering, CLAIB 2014
País/Territorio	Argentina
Ciudad	Paraná
Período	29/10/14 → 31/10/14

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10.1007/978-3-319-13117-7_15

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Pérez-Vielma, N. M., Ibarra-García, G. D., Gómez-López, M., Martínez Godínez, M. A., González Martínez, H., González Díaz, C. A., Del-Río-Navarro, B. E., Martínez-Silva, V. F., Martínez-Rivas, A., Vargas Varval, A. V., & Miliar-García, A. (2015). Development of DNA biosensors for single nucleotide polymorphisms. En A. Braidot, & A. Hadad (Eds.), VI Latin American Congress on Biomedical Engineering, CLAIB 2014 (pp. 55-58). (IFMBE Proceedings; Vol. 49). Springer Verlag. https://doi.org/10.1007/978-3-319-13117-7_15

@inproceedings{711fb7ac6a4b4543b147eb8ae67ef18c,

title = "Development of DNA biosensors for single nucleotide polymorphisms",

abstract = "The development of a DNA biosensor for the detection of any single nucleotide polymorphism (SNP) implemented on the basis of a quartz crystal resonator as transducer is presented. The methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C SNPs were chosen to perform a proof of concept. We designed and proved a specific surface chemistry on gold in order to use human DNA with no chemical modification as probe. The immobilization of the human DNA via the proposed surface chemistry was successfully characterized with an Atomic Force Microscope (AFM). The DNA biosensor proposed here results in a specific and rapid manner to detect the evaluated SNP.",

keywords = "DNA biosensors, Methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C single nucleotide polymorphisms (SNPs), Quartz crystal microbalance (QCM)",

author = "P{\'e}rez-Vielma, {N. M.} and Ibarra-Garc{\'i}a, {G. D.} and M. G{\'o}mez-L{\'o}pez and {Mart{\'i}nez God{\'i}nez}, {M. A.} and {Gonz{\'a}lez Mart{\'i}nez}, H. and {Gonz{\'a}lez D{\'i}az}, {C. A.} and Del-R{\'i}o-Navarro, {B. E.} and Mart{\'i}nez-Silva, {V. F.} and A. Mart{\'i}nez-Rivas and {Vargas Varval}, {A. V.} and A. Miliar-Garc{\'i}a",

note = "Publisher Copyright: {\textcopyright} Springer International Publishing Switzerland 2015.; 6th Latin American Congress on Biomedical Engineering, CLAIB 2014 ; Conference date: 29-10-2014 Through 31-10-2014",

year = "2015",

doi = "10.1007/978-3-319-13117-7_15",

language = "Ingl{\'e}s",

series = "IFMBE Proceedings",

publisher = "Springer Verlag",

pages = "55--58",

editor = "Ariel Braidot and Alejandro Hadad",

booktitle = "VI Latin American Congress on Biomedical Engineering, CLAIB 2014",

address = "Alemania",

}

Pérez-Vielma, NM, Ibarra-García, GD, Gómez-López, M , Martínez Godínez, MA , González Martínez, H , González Díaz, CA, Del-Río-Navarro, BE, Martínez-Silva, VF, Martínez-Rivas, A, Vargas Varval, AV & Miliar-García, A 2015, Development of DNA biosensors for single nucleotide polymorphisms. En A Braidot & A Hadad (eds.), VI Latin American Congress on Biomedical Engineering, CLAIB 2014. IFMBE Proceedings, vol. 49, Springer Verlag, pp. 55-58, 6th Latin American Congress on Biomedical Engineering, CLAIB 2014, Paraná, Argentina, 29/10/14. https://doi.org/10.1007/978-3-319-13117-7_15

Development of DNA biosensors for single nucleotide polymorphisms. / Pérez-Vielma, N. M.; Ibarra-García, G. D.; Gómez-López, M. et al.
VI Latin American Congress on Biomedical Engineering, CLAIB 2014. ed. / Ariel Braidot; Alejandro Hadad. Springer Verlag, 2015. p. 55-58 (IFMBE Proceedings; Vol. 49).

Producción científica: Capítulo del libro/informe/acta de congreso › Contribución a la conferencia › revisión exhaustiva

TY - GEN

T1 - Development of DNA biosensors for single nucleotide polymorphisms

AU - Pérez-Vielma, N. M.

AU - Ibarra-García, G. D.

AU - Gómez-López, M.

AU - Martínez Godínez, M. A.

AU - González Martínez, H.

AU - González Díaz, C. A.

AU - Del-Río-Navarro, B. E.

AU - Martínez-Silva, V. F.

AU - Martínez-Rivas, A.

AU - Vargas Varval, A. V.

AU - Miliar-García, A.

PY - 2015

Y1 - 2015

N2 - The development of a DNA biosensor for the detection of any single nucleotide polymorphism (SNP) implemented on the basis of a quartz crystal resonator as transducer is presented. The methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C SNPs were chosen to perform a proof of concept. We designed and proved a specific surface chemistry on gold in order to use human DNA with no chemical modification as probe. The immobilization of the human DNA via the proposed surface chemistry was successfully characterized with an Atomic Force Microscope (AFM). The DNA biosensor proposed here results in a specific and rapid manner to detect the evaluated SNP.

AB - The development of a DNA biosensor for the detection of any single nucleotide polymorphism (SNP) implemented on the basis of a quartz crystal resonator as transducer is presented. The methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C SNPs were chosen to perform a proof of concept. We designed and proved a specific surface chemistry on gold in order to use human DNA with no chemical modification as probe. The immobilization of the human DNA via the proposed surface chemistry was successfully characterized with an Atomic Force Microscope (AFM). The DNA biosensor proposed here results in a specific and rapid manner to detect the evaluated SNP.

KW - DNA biosensors

KW - Methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C single nucleotide polymorphisms (SNPs)

KW - Quartz crystal microbalance (QCM)

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U2 - 10.1007/978-3-319-13117-7_15

DO - 10.1007/978-3-319-13117-7_15

M3 - Contribución a la conferencia

AN - SCOPUS:84925258511

T3 - IFMBE Proceedings

SP - 55

EP - 58

BT - VI Latin American Congress on Biomedical Engineering, CLAIB 2014

A2 - Braidot, Ariel

A2 - Hadad, Alejandro

PB - Springer Verlag

T2 - 6th Latin American Congress on Biomedical Engineering, CLAIB 2014

Y2 - 29 October 2014 through 31 October 2014

ER -

Pérez-Vielma NM, Ibarra-García GD, Gómez-López M , Martínez Godínez MA , González Martínez H , González Díaz CA et al. Development of DNA biosensors for single nucleotide polymorphisms. En Braidot A, Hadad A, editores, VI Latin American Congress on Biomedical Engineering, CLAIB 2014. Springer Verlag. 2015. p. 55-58. (IFMBE Proceedings). doi: 10.1007/978-3-319-13117-7_15

Development of DNA biosensors for single nucleotide polymorphisms

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