Development of DNA biosensors for single nucleotide polymorphisms

N. M. Pérez-Vielma; G. D. Ibarra-García; M. Gómez-López; M. A. Martínez Godínez; H. González Martínez; C. A. González Díaz; B. E. Del-Río-Navarro; V. F. Martínez-Silva; A. Martínez-Rivas; A. V. Vargas Varval; A. Miliar-García

doi:10.1007/978-3-319-13117-7_15

Development of DNA biosensors for single nucleotide polymorphisms

N. M. Pérez-Vielma, G. D. Ibarra-García, M. Gómez-López, M. A. Martínez Godínez, H. González Martínez, C. A. González Díaz, B. E. Del-Río-Navarro, V. F. Martínez-Silva, A. Martínez-Rivas, A. V. Vargas Varval, A. Miliar-García

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The development of a DNA biosensor for the detection of any single nucleotide polymorphism (SNP) implemented on the basis of a quartz crystal resonator as transducer is presented. The methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C SNPs were chosen to perform a proof of concept. We designed and proved a specific surface chemistry on gold in order to use human DNA with no chemical modification as probe. The immobilization of the human DNA via the proposed surface chemistry was successfully characterized with an Atomic Force Microscope (AFM). The DNA biosensor proposed here results in a specific and rapid manner to detect the evaluated SNP.

Original language	English
Title of host publication	VI Latin American Congress on Biomedical Engineering, CLAIB 2014
Editors	Ariel Braidot, Alejandro Hadad
Publisher	Springer Verlag
Pages	55-58
Number of pages	4
ISBN (Electronic)	9783319131160
DOIs	https://doi.org/10.1007/978-3-319-13117-7_15
State	Published - 2015
Event	6th Latin American Congress on Biomedical Engineering, CLAIB 2014 - Paraná, Argentina Duration: 29 Oct 2014 → 31 Oct 2014

Publication series

Name	IFMBE Proceedings
Volume	49
ISSN (Print)	1680-0737

Conference

Conference	6th Latin American Congress on Biomedical Engineering, CLAIB 2014
Country/Territory	Argentina
City	Paraná
Period	29/10/14 → 31/10/14

Keywords

DNA biosensors
Methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C single nucleotide polymorphisms (SNPs)
Quartz crystal microbalance (QCM)

Access to Document

10.1007/978-3-319-13117-7_15

Cite this

Pérez-Vielma, N. M., Ibarra-García, G. D., Gómez-López, M., Martínez Godínez, M. A., González Martínez, H., González Díaz, C. A., Del-Río-Navarro, B. E., Martínez-Silva, V. F., Martínez-Rivas, A., Vargas Varval, A. V., & Miliar-García, A. (2015). Development of DNA biosensors for single nucleotide polymorphisms. In A. Braidot, & A. Hadad (Eds.), VI Latin American Congress on Biomedical Engineering, CLAIB 2014 (pp. 55-58). (IFMBE Proceedings; Vol. 49). Springer Verlag. https://doi.org/10.1007/978-3-319-13117-7_15

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title = "Development of DNA biosensors for single nucleotide polymorphisms",

abstract = "The development of a DNA biosensor for the detection of any single nucleotide polymorphism (SNP) implemented on the basis of a quartz crystal resonator as transducer is presented. The methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C SNPs were chosen to perform a proof of concept. We designed and proved a specific surface chemistry on gold in order to use human DNA with no chemical modification as probe. The immobilization of the human DNA via the proposed surface chemistry was successfully characterized with an Atomic Force Microscope (AFM). The DNA biosensor proposed here results in a specific and rapid manner to detect the evaluated SNP.",

keywords = "DNA biosensors, Methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C single nucleotide polymorphisms (SNPs), Quartz crystal microbalance (QCM)",

author = "P{\'e}rez-Vielma, {N. M.} and Ibarra-Garc{\'i}a, {G. D.} and M. G{\'o}mez-L{\'o}pez and {Mart{\'i}nez God{\'i}nez}, {M. A.} and {Gonz{\'a}lez Mart{\'i}nez}, H. and {Gonz{\'a}lez D{\'i}az}, {C. A.} and Del-R{\'i}o-Navarro, {B. E.} and Mart{\'i}nez-Silva, {V. F.} and A. Mart{\'i}nez-Rivas and {Vargas Varval}, {A. V.} and A. Miliar-Garc{\'i}a",

note = "Publisher Copyright: {\textcopyright} Springer International Publishing Switzerland 2015.; 6th Latin American Congress on Biomedical Engineering, CLAIB 2014 ; Conference date: 29-10-2014 Through 31-10-2014",

year = "2015",

doi = "10.1007/978-3-319-13117-7_15",

language = "Ingl{\'e}s",

series = "IFMBE Proceedings",

publisher = "Springer Verlag",

pages = "55--58",

editor = "Ariel Braidot and Alejandro Hadad",

booktitle = "VI Latin American Congress on Biomedical Engineering, CLAIB 2014",

address = "Alemania",

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Pérez-Vielma, NM, Ibarra-García, GD, Gómez-López, M , Martínez Godínez, MA , González Martínez, H , González Díaz, CA, Del-Río-Navarro, BE, Martínez-Silva, VF, Martínez-Rivas, A, Vargas Varval, AV & Miliar-García, A 2015, Development of DNA biosensors for single nucleotide polymorphisms. in A Braidot & A Hadad (eds), VI Latin American Congress on Biomedical Engineering, CLAIB 2014. IFMBE Proceedings, vol. 49, Springer Verlag, pp. 55-58, 6th Latin American Congress on Biomedical Engineering, CLAIB 2014, Paraná, Argentina, 29/10/14. https://doi.org/10.1007/978-3-319-13117-7_15

Development of DNA biosensors for single nucleotide polymorphisms. / Pérez-Vielma, N. M.; Ibarra-García, G. D.; Gómez-López, M. et al.
VI Latin American Congress on Biomedical Engineering, CLAIB 2014. ed. / Ariel Braidot; Alejandro Hadad. Springer Verlag, 2015. p. 55-58 (IFMBE Proceedings; Vol. 49).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Development of DNA biosensors for single nucleotide polymorphisms

AU - Pérez-Vielma, N. M.

AU - Ibarra-García, G. D.

AU - Gómez-López, M.

AU - Martínez Godínez, M. A.

AU - González Martínez, H.

AU - González Díaz, C. A.

AU - Del-Río-Navarro, B. E.

AU - Martínez-Silva, V. F.

AU - Martínez-Rivas, A.

AU - Vargas Varval, A. V.

AU - Miliar-García, A.

PY - 2015

Y1 - 2015

N2 - The development of a DNA biosensor for the detection of any single nucleotide polymorphism (SNP) implemented on the basis of a quartz crystal resonator as transducer is presented. The methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C SNPs were chosen to perform a proof of concept. We designed and proved a specific surface chemistry on gold in order to use human DNA with no chemical modification as probe. The immobilization of the human DNA via the proposed surface chemistry was successfully characterized with an Atomic Force Microscope (AFM). The DNA biosensor proposed here results in a specific and rapid manner to detect the evaluated SNP.

AB - The development of a DNA biosensor for the detection of any single nucleotide polymorphism (SNP) implemented on the basis of a quartz crystal resonator as transducer is presented. The methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C SNPs were chosen to perform a proof of concept. We designed and proved a specific surface chemistry on gold in order to use human DNA with no chemical modification as probe. The immobilization of the human DNA via the proposed surface chemistry was successfully characterized with an Atomic Force Microscope (AFM). The DNA biosensor proposed here results in a specific and rapid manner to detect the evaluated SNP.

KW - DNA biosensors

KW - Methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C single nucleotide polymorphisms (SNPs)

KW - Quartz crystal microbalance (QCM)

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U2 - 10.1007/978-3-319-13117-7_15

DO - 10.1007/978-3-319-13117-7_15

M3 - Contribución a la conferencia

AN - SCOPUS:84925258511

T3 - IFMBE Proceedings

SP - 55

EP - 58

BT - VI Latin American Congress on Biomedical Engineering, CLAIB 2014

A2 - Braidot, Ariel

A2 - Hadad, Alejandro

PB - Springer Verlag

T2 - 6th Latin American Congress on Biomedical Engineering, CLAIB 2014

Y2 - 29 October 2014 through 31 October 2014

ER -

Pérez-Vielma NM, Ibarra-García GD, Gómez-López M , Martínez Godínez MA , González Martínez H , González Díaz CA et al. Development of DNA biosensors for single nucleotide polymorphisms. In Braidot A, Hadad A, editors, VI Latin American Congress on Biomedical Engineering, CLAIB 2014. Springer Verlag. 2015. p. 55-58. (IFMBE Proceedings). doi: 10.1007/978-3-319-13117-7_15

Development of DNA biosensors for single nucleotide polymorphisms

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