TY - JOUR
T1 - Cytotoxic constituents from Daphnia pulex
AU - Pérez Gutierrez, Rosa Martha
AU - Lule P, R.
PY - 2005/6
Y1 - 2005/6
N2 - Activity-guided fractionation of the methanol extract of Daphnia pulex (Leydig) using the MTT assay and P-388, A549, KB, and HT-29 cell lines permitted the isolation of four known glyceroglycolipid compounds: (2S)-1-O-(7Z,1OZ,13Z)- hexadecatrienoyl-2-O-(7Z,1OZ)-hexadecadienoyl-3-O-β-D-galactopyranosyl-sn- glycerol, (2S)-1-O-(7Z,10Z)-hexadecadienoyl-2-O-(7Z,10Z)-hexadecadienoyl-3-O- β-D-galactopyranosyl-sn-glycerol, (2S)-1-O-(7Z,10Z)-hexadecadienolyl-2-O- linolenoyl-3-O-β-D-galactopyranosyl-sn-glycerol, and (2S)-1-O-(9Z, 12Z)-octadecadienoyl-2-O-(1Z,10Z)-hexadecadienoyl-3-O-(α-D- galactopyranosyl(1-6)-β-D-galactopyranosyl-sn-glycerol. All the isolates were found to be moderately cytotoxic (ED50 < 4 μg/ml) toward P-388, A549, KB, and HT-29 cell lines.
AB - Activity-guided fractionation of the methanol extract of Daphnia pulex (Leydig) using the MTT assay and P-388, A549, KB, and HT-29 cell lines permitted the isolation of four known glyceroglycolipid compounds: (2S)-1-O-(7Z,1OZ,13Z)- hexadecatrienoyl-2-O-(7Z,1OZ)-hexadecadienoyl-3-O-β-D-galactopyranosyl-sn- glycerol, (2S)-1-O-(7Z,10Z)-hexadecadienoyl-2-O-(7Z,10Z)-hexadecadienoyl-3-O- β-D-galactopyranosyl-sn-glycerol, (2S)-1-O-(7Z,10Z)-hexadecadienolyl-2-O- linolenoyl-3-O-β-D-galactopyranosyl-sn-glycerol, and (2S)-1-O-(9Z, 12Z)-octadecadienoyl-2-O-(1Z,10Z)-hexadecadienoyl-3-O-(α-D- galactopyranosyl(1-6)-β-D-galactopyranosyl-sn-glycerol. All the isolates were found to be moderately cytotoxic (ED50 < 4 μg/ml) toward P-388, A549, KB, and HT-29 cell lines.
KW - Cytotoxic activity
KW - Daphnia pulex
KW - Digalactosyl diacylglycerols
KW - Monogalactosyl diacylglycerols
UR - http://www.scopus.com/inward/record.url?scp=22644436962&partnerID=8YFLogxK
U2 - 10.1080/13880200590951702
DO - 10.1080/13880200590951702
M3 - Artículo
SN - 1388-0209
VL - 43
SP - 313
EP - 316
JO - Pharmaceutical Biology
JF - Pharmaceutical Biology
IS - 4
ER -