Cytotoxic constituents from Daphnia pulex

Rosa Martha Pérez Gutierrez; R. Lule P

doi:10.1080/13880200590951702

Cytotoxic constituents from Daphnia pulex

Rosa Martha Pérez Gutierrez, R. Lule P

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Activity-guided fractionation of the methanol extract of Daphnia pulex (Leydig) using the MTT assay and P-388, A549, KB, and HT-29 cell lines permitted the isolation of four known glyceroglycolipid compounds: (2S)-1-O-(7Z,1OZ,13Z)- hexadecatrienoyl-2-O-(7Z,1OZ)-hexadecadienoyl-3-O-β-D-galactopyranosyl-sn- glycerol, (2S)-1-O-(7Z,10Z)-hexadecadienoyl-2-O-(7Z,10Z)-hexadecadienoyl-3-O- β-D-galactopyranosyl-sn-glycerol, (2S)-1-O-(7Z,10Z)-hexadecadienolyl-2-O- linolenoyl-3-O-β-D-galactopyranosyl-sn-glycerol, and (2S)-1-O-(9Z, 12Z)-octadecadienoyl-2-O-(1Z,10Z)-hexadecadienoyl-3-O-(α-D- galactopyranosyl(1-6)-β-D-galactopyranosyl-sn-glycerol. All the isolates were found to be moderately cytotoxic (ED₅₀ < 4 μg/ml) toward P-388, A549, KB, and HT-29 cell lines.

Original language	English
Pages (from-to)	313-316
Number of pages	4
Journal	Pharmaceutical Biology
Volume	43
Issue number	4
DOIs	https://doi.org/10.1080/13880200590951702
State	Published - Jun 2005
Externally published	Yes

Keywords

Cytotoxic activity
Daphnia pulex
Digalactosyl diacylglycerols
Monogalactosyl diacylglycerols

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10.1080/13880200590951702

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title = "Cytotoxic constituents from Daphnia pulex",

abstract = "Activity-guided fractionation of the methanol extract of Daphnia pulex (Leydig) using the MTT assay and P-388, A549, KB, and HT-29 cell lines permitted the isolation of four known glyceroglycolipid compounds: (2S)-1-O-(7Z,1OZ,13Z)- hexadecatrienoyl-2-O-(7Z,1OZ)-hexadecadienoyl-3-O-β-D-galactopyranosyl-sn- glycerol, (2S)-1-O-(7Z,10Z)-hexadecadienoyl-2-O-(7Z,10Z)-hexadecadienoyl-3-O- β-D-galactopyranosyl-sn-glycerol, (2S)-1-O-(7Z,10Z)-hexadecadienolyl-2-O- linolenoyl-3-O-β-D-galactopyranosyl-sn-glycerol, and (2S)-1-O-(9Z, 12Z)-octadecadienoyl-2-O-(1Z,10Z)-hexadecadienoyl-3-O-(α-D- galactopyranosyl(1-6)-β-D-galactopyranosyl-sn-glycerol. All the isolates were found to be moderately cytotoxic (ED50 < 4 μg/ml) toward P-388, A549, KB, and HT-29 cell lines.",

keywords = "Cytotoxic activity, Daphnia pulex, Digalactosyl diacylglycerols, Monogalactosyl diacylglycerols",

author = "{P{\'e}rez Gutierrez}, {Rosa Martha} and {Lule P}, R.",

year = "2005",

month = jun,

doi = "10.1080/13880200590951702",

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volume = "43",

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journal = "Pharmaceutical Biology",

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AU - Pérez Gutierrez, Rosa Martha

AU - Lule P, R.

PY - 2005/6

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AB - Activity-guided fractionation of the methanol extract of Daphnia pulex (Leydig) using the MTT assay and P-388, A549, KB, and HT-29 cell lines permitted the isolation of four known glyceroglycolipid compounds: (2S)-1-O-(7Z,1OZ,13Z)- hexadecatrienoyl-2-O-(7Z,1OZ)-hexadecadienoyl-3-O-β-D-galactopyranosyl-sn- glycerol, (2S)-1-O-(7Z,10Z)-hexadecadienoyl-2-O-(7Z,10Z)-hexadecadienoyl-3-O- β-D-galactopyranosyl-sn-glycerol, (2S)-1-O-(7Z,10Z)-hexadecadienolyl-2-O- linolenoyl-3-O-β-D-galactopyranosyl-sn-glycerol, and (2S)-1-O-(9Z, 12Z)-octadecadienoyl-2-O-(1Z,10Z)-hexadecadienoyl-3-O-(α-D- galactopyranosyl(1-6)-β-D-galactopyranosyl-sn-glycerol. All the isolates were found to be moderately cytotoxic (ED50 < 4 μg/ml) toward P-388, A549, KB, and HT-29 cell lines.

KW - Cytotoxic activity

KW - Daphnia pulex

KW - Digalactosyl diacylglycerols

KW - Monogalactosyl diacylglycerols

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DO - 10.1080/13880200590951702

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JO - Pharmaceutical Biology

JF - Pharmaceutical Biology

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ER -

Cytotoxic constituents from Daphnia pulex

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Keywords

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