TY - JOUR
T1 - Cyanuric acid biodegradation by a mixed bacterial culture of Agrobacterium tumefaciens and Acinetobacter sp. in a packed bed biofilm reactor
AU - Galíndez-Nájera, S. P.
AU - Llamas-Martínez, M. A.
AU - Ruiz-Ordaz, N.
AU - Juárez-Ramírez, C.
AU - Mondragón-Parada, M. E.
AU - Ahuatzi-Chacón, D.
AU - Galíndez-Mayer, J.
N1 - Funding Information:
S.P. Galíndez-Nájera and M.A. Llamas-Martínez, are holders of a research grant from PIFI-IPN. C. Juárez-Ramírez, N. Ruiz-Ordaz, D. Ahuatzi-Chacón, and J. Galíndez-Mayer, are holders of grants from COFAA-IPN, SIP-IPN, and SNI-Conacyt.
PY - 2009/2
Y1 - 2009/2
N2 - Cyanuric acid (1,3,5-triazine-2,4,6-triol [OOOT]) is a common biodegradation byproduct of triazinic herbicides, frequently accumulated in soils or water when supplementary carbon sources are absent. A binary bacterial culture able to degrade OOOT was selected through a continuous selection process accomplished in a chemostat fed with a mineral salt (MS) medium containing cyanuric acid as the sole carbon and nitrogen source. By sequence comparison of their 16S rDNA amplicons, bacterial strains were identified as Agrobacterium tumefaciens, and Acinetobacter sp. When the binary culture immobilized in a packed bed reactor (PBR) was fed with MS medium containing OOOT (50 mg L -1), its removal efficiencies were about 95%; when it was fed with OOOT plus glucose (120 mg L-1) as a supplementary carbon source, its removal efficiencies were closer to 100%. From sessile cells, attached to PBR porous support, or free cells present in the outflowing medium, DNA was extracted and used for Random Amplification of Polymorphic DNA analysis. Electrophoretic patterns obtained were compared to those of pure bacterial strains, a clear predominance of A. tumefaciens in PBR was observed. Although in continuous suspended cell culture, a stable binary community could be maintained, the attachment capability of A. tumefaciens represented a selective advantage over Acinetobacter sp. in the biofilm reactor, favoring its predominance in the porous stone support.
AB - Cyanuric acid (1,3,5-triazine-2,4,6-triol [OOOT]) is a common biodegradation byproduct of triazinic herbicides, frequently accumulated in soils or water when supplementary carbon sources are absent. A binary bacterial culture able to degrade OOOT was selected through a continuous selection process accomplished in a chemostat fed with a mineral salt (MS) medium containing cyanuric acid as the sole carbon and nitrogen source. By sequence comparison of their 16S rDNA amplicons, bacterial strains were identified as Agrobacterium tumefaciens, and Acinetobacter sp. When the binary culture immobilized in a packed bed reactor (PBR) was fed with MS medium containing OOOT (50 mg L -1), its removal efficiencies were about 95%; when it was fed with OOOT plus glucose (120 mg L-1) as a supplementary carbon source, its removal efficiencies were closer to 100%. From sessile cells, attached to PBR porous support, or free cells present in the outflowing medium, DNA was extracted and used for Random Amplification of Polymorphic DNA analysis. Electrophoretic patterns obtained were compared to those of pure bacterial strains, a clear predominance of A. tumefaciens in PBR was observed. Although in continuous suspended cell culture, a stable binary community could be maintained, the attachment capability of A. tumefaciens represented a selective advantage over Acinetobacter sp. in the biofilm reactor, favoring its predominance in the porous stone support.
UR - http://www.scopus.com/inward/record.url?scp=85042596012&partnerID=8YFLogxK
U2 - 10.1007/s10295-008-0496-5
DO - 10.1007/s10295-008-0496-5
M3 - Artículo
SN - 1367-5435
VL - 36
SP - 275
EP - 284
JO - Journal of Industrial Microbiology and Biotechnology
JF - Journal of Industrial Microbiology and Biotechnology
IS - 2
ER -