Cyanuric acid biodegradation by a mixed bacterial culture of Agrobacterium tumefaciens and Acinetobacter sp. in a packed bed biofilm reactor

S. P. Galíndez-Nájera, M. A. Llamas-Martínez, N. Ruiz-Ordaz, C. Juárez-Ramírez, M. E. Mondragón-Parada, D. Ahuatzi-Chacón, J. Galíndez-Mayer

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cyanuric acid (1,3,5-triazine-2,4,6-triol [OOOT]) is a common biodegradation byproduct of triazinic herbicides, frequently accumulated in soils or water when supplementary carbon sources are absent. A binary bacterial culture able to degrade OOOT was selected through a continuous selection process accomplished in a chemostat fed with a mineral salt (MS) medium containing cyanuric acid as the sole carbon and nitrogen source. By sequence comparison of their 16S rDNA amplicons, bacterial strains were identified as Agrobacterium tumefaciens, and Acinetobacter sp. When the binary culture immobilized in a packed bed reactor (PBR) was fed with MS medium containing OOOT (50 mg L -1), its removal efficiencies were about 95%; when it was fed with OOOT plus glucose (120 mg L-1) as a supplementary carbon source, its removal efficiencies were closer to 100%. From sessile cells, attached to PBR porous support, or free cells present in the outflowing medium, DNA was extracted and used for Random Amplification of Polymorphic DNA analysis. Electrophoretic patterns obtained were compared to those of pure bacterial strains, a clear predominance of A. tumefaciens in PBR was observed. Although in continuous suspended cell culture, a stable binary community could be maintained, the attachment capability of A. tumefaciens represented a selective advantage over Acinetobacter sp. in the biofilm reactor, favoring its predominance in the porous stone support. © Society for Industrial Microbiology 2008.
Original languageAmerican English
Pages (from-to)275-284
Number of pages246
JournalJournal of Industrial Microbiology and Biotechnology
DOIs
StatePublished - 1 Jan 2009

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Agrobacterium tumefaciens
Acinetobacter
Biofilms
Packed beds
Biodegradation
Carbon
Industrial microbiology
Minerals
Industrial Microbiology
Acids
DNA
Salts
Chemostats
Triazines
Herbicides
Ribosomal DNA
Cell culture
Amplification
Byproducts
Glucose

Cite this

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title = "Cyanuric acid biodegradation by a mixed bacterial culture of Agrobacterium tumefaciens and Acinetobacter sp. in a packed bed biofilm reactor",
abstract = "Cyanuric acid (1,3,5-triazine-2,4,6-triol [OOOT]) is a common biodegradation byproduct of triazinic herbicides, frequently accumulated in soils or water when supplementary carbon sources are absent. A binary bacterial culture able to degrade OOOT was selected through a continuous selection process accomplished in a chemostat fed with a mineral salt (MS) medium containing cyanuric acid as the sole carbon and nitrogen source. By sequence comparison of their 16S rDNA amplicons, bacterial strains were identified as Agrobacterium tumefaciens, and Acinetobacter sp. When the binary culture immobilized in a packed bed reactor (PBR) was fed with MS medium containing OOOT (50 mg L -1), its removal efficiencies were about 95{\%}; when it was fed with OOOT plus glucose (120 mg L-1) as a supplementary carbon source, its removal efficiencies were closer to 100{\%}. From sessile cells, attached to PBR porous support, or free cells present in the outflowing medium, DNA was extracted and used for Random Amplification of Polymorphic DNA analysis. Electrophoretic patterns obtained were compared to those of pure bacterial strains, a clear predominance of A. tumefaciens in PBR was observed. Although in continuous suspended cell culture, a stable binary community could be maintained, the attachment capability of A. tumefaciens represented a selective advantage over Acinetobacter sp. in the biofilm reactor, favoring its predominance in the porous stone support. {\circledC} Society for Industrial Microbiology 2008.",
author = "Gal{\'i}ndez-N{\'a}jera, {S. P.} and Llamas-Mart{\'i}nez, {M. A.} and N. Ruiz-Ordaz and C. Ju{\'a}rez-Ram{\'i}rez and Mondrag{\'o}n-Parada, {M. E.} and D. Ahuatzi-Chac{\'o}n and J. Gal{\'i}ndez-Mayer",
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Cyanuric acid biodegradation by a mixed bacterial culture of Agrobacterium tumefaciens and Acinetobacter sp. in a packed bed biofilm reactor. / Galíndez-Nájera, S. P.; Llamas-Martínez, M. A.; Ruiz-Ordaz, N.; Juárez-Ramírez, C.; Mondragón-Parada, M. E.; Ahuatzi-Chacón, D.; Galíndez-Mayer, J.

In: Journal of Industrial Microbiology and Biotechnology, 01.01.2009, p. 275-284.

Research output: Contribution to journalArticle

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AU - Galíndez-Nájera, S. P.

AU - Llamas-Martínez, M. A.

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AU - Ahuatzi-Chacón, D.

AU - Galíndez-Mayer, J.

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