TY - JOUR
T1 - Culture-induced variation in plants of Coffea arabica cv. caturra rojo, regenerated by direct and indirect somatic embryogenesis
AU - Sanchez-Teyer, L. Felipe
AU - Quiroz-Figueroa, Francisco
AU - Loyola-Vargas, Victor
AU - Infante, Diogenes
N1 - Funding Information:
The authors thank Miriam Monforte and Marcela Méndez for technical help. L. Felipe Sanchez-Teyer acknowledges his Ph. D. scholarships from the National Council for Science and Technology of Mexico (CONACYT). This work was supported by CONACYT grants Nos. 4123P-N and 31816-N.
PY - 2003/2
Y1 - 2003/2
N2 - Amplified fragment-length polymorphism (AFLP) was used to evaluate the stability of DNA in regenerated plantlets of Coffea arabica obtained by direct (DSE) and indirect somatic embryogenesis (ISE). Cluster analysis using the unweighted pair-group method (UPGMA), showed no specific grouping pattern related to the type of embryogenesis. These results suggest that the somatic embryogenesis (SE) process has a mechanism for the selection of normal and competent cells. Bulked DNA from regenerated plants obtained by DSE and ISE, and from the mother plants, was used to characterize specific AFLP fragments associated with each SE process. Twenty-three primer combinations were tested. A total of 1446 bands were analyzed, with 11.4% being polymorphic and 84% being specific for regenerated plants. Furthermore, specific bands were detected for DSE, ISE, and the mother plants. These results indicate that the SE process induces rearrangements at the DNA level and demonstrates discrepancies between the mechanisms involved in each SE process. Coffea arabica breeding programs that involve DSE and ISE can use AFLP as an additional tool for assessing DNA stability.
AB - Amplified fragment-length polymorphism (AFLP) was used to evaluate the stability of DNA in regenerated plantlets of Coffea arabica obtained by direct (DSE) and indirect somatic embryogenesis (ISE). Cluster analysis using the unweighted pair-group method (UPGMA), showed no specific grouping pattern related to the type of embryogenesis. These results suggest that the somatic embryogenesis (SE) process has a mechanism for the selection of normal and competent cells. Bulked DNA from regenerated plants obtained by DSE and ISE, and from the mother plants, was used to characterize specific AFLP fragments associated with each SE process. Twenty-three primer combinations were tested. A total of 1446 bands were analyzed, with 11.4% being polymorphic and 84% being specific for regenerated plants. Furthermore, specific bands were detected for DSE, ISE, and the mother plants. These results indicate that the SE process induces rearrangements at the DNA level and demonstrates discrepancies between the mechanisms involved in each SE process. Coffea arabica breeding programs that involve DSE and ISE can use AFLP as an additional tool for assessing DNA stability.
KW - AFLP
KW - Bulk analysis
KW - Cluster analysis
KW - Coffea arabica
KW - Genetic variation
KW - Molecular markers
UR - http://www.scopus.com/inward/record.url?scp=0037322191&partnerID=8YFLogxK
U2 - 10.1385/MB:23:2:107
DO - 10.1385/MB:23:2:107
M3 - Artículo
SN - 1073-6085
VL - 23
SP - 107
EP - 115
JO - Molecular Biotechnology
JF - Molecular Biotechnology
IS - 2
ER -