Culture-induced variation in plants of Coffea arabica cv. caturra rojo, regenerated by direct and indirect somatic embryogenesis

L. Felipe Sanchez-Teyer, Francisco Quiroz-Figueroa, Victor Loyola-Vargas, Diogenes Infante

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Amplified fragment-length polymorphism (AFLP) was used to evaluate the stability of DNA in regenerated plantlets of Coffea arabica obtained by direct (DSE) and indirect somatic embryogenesis (ISE). Cluster analysis using the unweighted pair-group method (UPGMA), showed no specific grouping pattern related to the type of embryogenesis. These results suggest that the somatic embryogenesis (SE) process has a mechanism for the selection of normal and competent cells. Bulked DNA from regenerated plants obtained by DSE and ISE, and from the mother plants, was used to characterize specific AFLP fragments associated with each SE process. Twenty-three primer combinations were tested. A total of 1446 bands were analyzed, with 11.4% being polymorphic and 84% being specific for regenerated plants. Furthermore, specific bands were detected for DSE, ISE, and the mother plants. These results indicate that the SE process induces rearrangements at the DNA level and demonstrates discrepancies between the mechanisms involved in each SE process. Coffea arabica breeding programs that involve DSE and ISE can use AFLP as an additional tool for assessing DNA stability.
Original languageAmerican English
Pages (from-to)107-115
Number of pages9
JournalApplied Biochemistry and Biotechnology - Part B Molecular Biotechnology
DOIs
StatePublished - 1 Feb 2003
Externally publishedYes

Fingerprint Dive into the research topics of 'Culture-induced variation in plants of Coffea arabica cv. caturra rojo, regenerated by direct and indirect somatic embryogenesis'. Together they form a unique fingerprint.

  • Cite this