TY - GEN
T1 - Biosensor of Inflammation Biomarker NLRP3 Based on Electrical Bioimpedance
AU - Gómez-López, Modesto
AU - Miliar-García, Ángel
AU - Pérez-Vielma, Nadia Mabel
AU - Ames-Lastra, G.
AU - Lara-Padilla, Eleazar
AU - Chávez, Katia Verónica
AU - García, Arianne Ruby
AU - Delgado, Josemaría
AU - Cortés, Ernesto
AU - González Díaz, César Antonio
N1 - Publisher Copyright:
© 2020, Springer Nature Switzerland AG.
PY - 2020
Y1 - 2020
N2 - The development of biosensors to identify the molecular markers of specific genes is fundamental for the implementation of new techniques that allow the detection of specific DNA sequences in a fast, economic, and simple way. Electrical Bioimpedance Spectroscopy (EBiS) has been used for the diagnosis and monitoring of human pathologies, and is recognized as a safe, fast, reusable, easy, and inexpensive technique. This study proves the development of a complementary DNA (cDNA) biosensor based on measurements of EBiS and of the immobilization of DNA without chemical modifications, and presents the evaluation of its potential usefulness in the detection of the gene expression of an inflammation characteristic biomarker, NLRP3, is presented. The obtained results demonstrate that EBiS can be used to identify different gene expression patterns, and measurements were compared with Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) and validated by Quantitative Polymerase Chain Reaction (qPCR). These results indicate the technical feasibility of a biosensor of specific genes through bioimpedance measurements in the immobilization of cDNA.
AB - The development of biosensors to identify the molecular markers of specific genes is fundamental for the implementation of new techniques that allow the detection of specific DNA sequences in a fast, economic, and simple way. Electrical Bioimpedance Spectroscopy (EBiS) has been used for the diagnosis and monitoring of human pathologies, and is recognized as a safe, fast, reusable, easy, and inexpensive technique. This study proves the development of a complementary DNA (cDNA) biosensor based on measurements of EBiS and of the immobilization of DNA without chemical modifications, and presents the evaluation of its potential usefulness in the detection of the gene expression of an inflammation characteristic biomarker, NLRP3, is presented. The obtained results demonstrate that EBiS can be used to identify different gene expression patterns, and measurements were compared with Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) and validated by Quantitative Polymerase Chain Reaction (qPCR). These results indicate the technical feasibility of a biosensor of specific genes through bioimpedance measurements in the immobilization of cDNA.
KW - Impedance
KW - QCM
KW - qPCR
UR - http://www.scopus.com/inward/record.url?scp=85075672302&partnerID=8YFLogxK
U2 - 10.1007/978-3-030-30648-9_191
DO - 10.1007/978-3-030-30648-9_191
M3 - Contribución a la conferencia
AN - SCOPUS:85075672302
SN - 9783030306472
T3 - IFMBE Proceedings
SP - 1488
EP - 1493
BT - 8th Latin American Conference on Biomedical Engineering and 42nd National Conference on Biomedical Engineering - Proceedings of CLAIB-CNIB 2019
A2 - González Díaz, César A.
A2 - Chapa González, Christian
A2 - Laciar Leber, Eric
A2 - Vélez, Hugo A.
A2 - Puente, Norma P.
A2 - Flores, Dora-Luz
A2 - Andrade, Adriano O.
A2 - Galván, Héctor A.
A2 - Martínez, Fabiola
A2 - García, Renato
A2 - Trujillo, Citlalli J.
A2 - Mejía, Aldo R.
PB - Springer
T2 - 8th Latin American Conference on Biomedical Engineering and the 42nd National Conference on Biomedical Engineering, CLAIB-CNIB 2019
Y2 - 2 October 2019 through 5 October 2019
ER -