Biosensor of Inflammation Biomarker NLRP3 Based on Electrical Bioimpedance

Modesto Gómez-López, Ángel Miliar-García, Nadia Mabel Pérez-Vielma, G. Ames-Lastra, Eleazar Lara-Padilla, Katia Verónica Chávez, Arianne Ruby García, Josemaría Delgado, Ernesto Cortés, César Antonio González Díaz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The development of biosensors to identify the molecular markers of specific genes is fundamental for the implementation of new techniques that allow the detection of specific DNA sequences in a fast, economic, and simple way. Electrical Bioimpedance Spectroscopy (EBiS) has been used for the diagnosis and monitoring of human pathologies, and is recognized as a safe, fast, reusable, easy, and inexpensive technique. This study proves the development of a complementary DNA (cDNA) biosensor based on measurements of EBiS and of the immobilization of DNA without chemical modifications, and presents the evaluation of its potential usefulness in the detection of the gene expression of an inflammation characteristic biomarker, NLRP3, is presented. The obtained results demonstrate that EBiS can be used to identify different gene expression patterns, and measurements were compared with Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) and validated by Quantitative Polymerase Chain Reaction (qPCR). These results indicate the technical feasibility of a biosensor of specific genes through bioimpedance measurements in the immobilization of cDNA.

Original languageEnglish
Title of host publication8th Latin American Conference on Biomedical Engineering and 42nd National Conference on Biomedical Engineering - Proceedings of CLAIB-CNIB 2019
EditorsCésar A. González Díaz, Christian Chapa González, Eric Laciar Leber, Hugo A. Vélez, Norma P. Puente, Dora-Luz Flores, Adriano O. Andrade, Héctor A. Galván, Fabiola Martínez, Renato García, Citlalli J. Trujillo, Aldo R. Mejía
PublisherSpringer
Pages1488-1493
Number of pages6
ISBN (Print)9783030306472
DOIs
StatePublished - 1 Jan 2020
Event8th Latin American Conference on Biomedical Engineering and the 42nd National Conference on Biomedical Engineering, CLAIB-CNIB 2019 - Cancún, Mexico
Duration: 2 Oct 20195 Oct 2019

Publication series

NameIFMBE Proceedings
Volume75
ISSN (Print)1680-0737
ISSN (Electronic)1433-9277

Conference

Conference8th Latin American Conference on Biomedical Engineering and the 42nd National Conference on Biomedical Engineering, CLAIB-CNIB 2019
CountryMexico
CityCancún
Period2/10/195/10/19

Fingerprint

Biomarkers
Biosensors
DNA
Spectroscopy
Gene expression
Complementary DNA
Genes
Monitoring
Quartz crystal microbalances
Polymerase chain reaction
DNA sequences
Chemical modification
Pathology
Economics

Keywords

  • Impedance
  • QCM
  • qPCR

Cite this

Gómez-López, M., Miliar-García, Á., Pérez-Vielma, N. M., Ames-Lastra, G., Lara-Padilla, E., Chávez, K. V., ... González Díaz, C. A. (2020). Biosensor of Inflammation Biomarker NLRP3 Based on Electrical Bioimpedance. In C. A. González Díaz, C. Chapa González, E. Laciar Leber, H. A. Vélez, N. P. Puente, D-L. Flores, A. O. Andrade, H. A. Galván, F. Martínez, R. García, C. J. Trujillo, ... A. R. Mejía (Eds.), 8th Latin American Conference on Biomedical Engineering and 42nd National Conference on Biomedical Engineering - Proceedings of CLAIB-CNIB 2019 (pp. 1488-1493). (IFMBE Proceedings; Vol. 75). Springer. https://doi.org/10.1007/978-3-030-30648-9_191
Gómez-López, Modesto ; Miliar-García, Ángel ; Pérez-Vielma, Nadia Mabel ; Ames-Lastra, G. ; Lara-Padilla, Eleazar ; Chávez, Katia Verónica ; García, Arianne Ruby ; Delgado, Josemaría ; Cortés, Ernesto ; González Díaz, César Antonio. / Biosensor of Inflammation Biomarker NLRP3 Based on Electrical Bioimpedance. 8th Latin American Conference on Biomedical Engineering and 42nd National Conference on Biomedical Engineering - Proceedings of CLAIB-CNIB 2019. editor / César A. González Díaz ; Christian Chapa González ; Eric Laciar Leber ; Hugo A. Vélez ; Norma P. Puente ; Dora-Luz Flores ; Adriano O. Andrade ; Héctor A. Galván ; Fabiola Martínez ; Renato García ; Citlalli J. Trujillo ; Aldo R. Mejía. Springer, 2020. pp. 1488-1493 (IFMBE Proceedings).
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title = "Biosensor of Inflammation Biomarker NLRP3 Based on Electrical Bioimpedance",
abstract = "The development of biosensors to identify the molecular markers of specific genes is fundamental for the implementation of new techniques that allow the detection of specific DNA sequences in a fast, economic, and simple way. Electrical Bioimpedance Spectroscopy (EBiS) has been used for the diagnosis and monitoring of human pathologies, and is recognized as a safe, fast, reusable, easy, and inexpensive technique. This study proves the development of a complementary DNA (cDNA) biosensor based on measurements of EBiS and of the immobilization of DNA without chemical modifications, and presents the evaluation of its potential usefulness in the detection of the gene expression of an inflammation characteristic biomarker, NLRP3, is presented. The obtained results demonstrate that EBiS can be used to identify different gene expression patterns, and measurements were compared with Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) and validated by Quantitative Polymerase Chain Reaction (qPCR). These results indicate the technical feasibility of a biosensor of specific genes through bioimpedance measurements in the immobilization of cDNA.",
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Gómez-López, M, Miliar-García, Á, Pérez-Vielma, NM, Ames-Lastra, G, Lara-Padilla, E, Chávez, KV, García, AR, Delgado, J, Cortés, E & González Díaz, CA 2020, Biosensor of Inflammation Biomarker NLRP3 Based on Electrical Bioimpedance. in CA González Díaz, C Chapa González, E Laciar Leber, HA Vélez, NP Puente, D-L Flores, AO Andrade, HA Galván, F Martínez, R García, CJ Trujillo & AR Mejía (eds), 8th Latin American Conference on Biomedical Engineering and 42nd National Conference on Biomedical Engineering - Proceedings of CLAIB-CNIB 2019. IFMBE Proceedings, vol. 75, Springer, pp. 1488-1493, 8th Latin American Conference on Biomedical Engineering and the 42nd National Conference on Biomedical Engineering, CLAIB-CNIB 2019, Cancún, Mexico, 2/10/19. https://doi.org/10.1007/978-3-030-30648-9_191

Biosensor of Inflammation Biomarker NLRP3 Based on Electrical Bioimpedance. / Gómez-López, Modesto; Miliar-García, Ángel; Pérez-Vielma, Nadia Mabel; Ames-Lastra, G.; Lara-Padilla, Eleazar; Chávez, Katia Verónica; García, Arianne Ruby; Delgado, Josemaría; Cortés, Ernesto; González Díaz, César Antonio.

8th Latin American Conference on Biomedical Engineering and 42nd National Conference on Biomedical Engineering - Proceedings of CLAIB-CNIB 2019. ed. / César A. González Díaz; Christian Chapa González; Eric Laciar Leber; Hugo A. Vélez; Norma P. Puente; Dora-Luz Flores; Adriano O. Andrade; Héctor A. Galván; Fabiola Martínez; Renato García; Citlalli J. Trujillo; Aldo R. Mejía. Springer, 2020. p. 1488-1493 (IFMBE Proceedings; Vol. 75).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Biosensor of Inflammation Biomarker NLRP3 Based on Electrical Bioimpedance

AU - Gómez-López, Modesto

AU - Miliar-García, Ángel

AU - Pérez-Vielma, Nadia Mabel

AU - Ames-Lastra, G.

AU - Lara-Padilla, Eleazar

AU - Chávez, Katia Verónica

AU - García, Arianne Ruby

AU - Delgado, Josemaría

AU - Cortés, Ernesto

AU - González Díaz, César Antonio

PY - 2020/1/1

Y1 - 2020/1/1

N2 - The development of biosensors to identify the molecular markers of specific genes is fundamental for the implementation of new techniques that allow the detection of specific DNA sequences in a fast, economic, and simple way. Electrical Bioimpedance Spectroscopy (EBiS) has been used for the diagnosis and monitoring of human pathologies, and is recognized as a safe, fast, reusable, easy, and inexpensive technique. This study proves the development of a complementary DNA (cDNA) biosensor based on measurements of EBiS and of the immobilization of DNA without chemical modifications, and presents the evaluation of its potential usefulness in the detection of the gene expression of an inflammation characteristic biomarker, NLRP3, is presented. The obtained results demonstrate that EBiS can be used to identify different gene expression patterns, and measurements were compared with Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) and validated by Quantitative Polymerase Chain Reaction (qPCR). These results indicate the technical feasibility of a biosensor of specific genes through bioimpedance measurements in the immobilization of cDNA.

AB - The development of biosensors to identify the molecular markers of specific genes is fundamental for the implementation of new techniques that allow the detection of specific DNA sequences in a fast, economic, and simple way. Electrical Bioimpedance Spectroscopy (EBiS) has been used for the diagnosis and monitoring of human pathologies, and is recognized as a safe, fast, reusable, easy, and inexpensive technique. This study proves the development of a complementary DNA (cDNA) biosensor based on measurements of EBiS and of the immobilization of DNA without chemical modifications, and presents the evaluation of its potential usefulness in the detection of the gene expression of an inflammation characteristic biomarker, NLRP3, is presented. The obtained results demonstrate that EBiS can be used to identify different gene expression patterns, and measurements were compared with Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) and validated by Quantitative Polymerase Chain Reaction (qPCR). These results indicate the technical feasibility of a biosensor of specific genes through bioimpedance measurements in the immobilization of cDNA.

KW - Impedance

KW - QCM

KW - qPCR

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BT - 8th Latin American Conference on Biomedical Engineering and 42nd National Conference on Biomedical Engineering - Proceedings of CLAIB-CNIB 2019

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A2 - Laciar Leber, Eric

A2 - Vélez, Hugo A.

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A2 - Andrade, Adriano O.

A2 - Galván, Héctor A.

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Gómez-López M, Miliar-García Á, Pérez-Vielma NM, Ames-Lastra G, Lara-Padilla E, Chávez KV et al. Biosensor of Inflammation Biomarker NLRP3 Based on Electrical Bioimpedance. In González Díaz CA, Chapa González C, Laciar Leber E, Vélez HA, Puente NP, Flores D-L, Andrade AO, Galván HA, Martínez F, García R, Trujillo CJ, Mejía AR, editors, 8th Latin American Conference on Biomedical Engineering and 42nd National Conference on Biomedical Engineering - Proceedings of CLAIB-CNIB 2019. Springer. 2020. p. 1488-1493. (IFMBE Proceedings). https://doi.org/10.1007/978-3-030-30648-9_191