TY - JOUR
T1 - Specific optical-based biosensor to rapid detection of salmonella typhimurium using FTIR
T2 - Evaluation in natural orange juice, as an application in food products
AU - Gómez-Montaño, F. J.
AU - Orduña-Díaz, A.
AU - Avelino-Flores, M. C.G.
AU - Avelino-Flores, F.
AU - Reyes-Betanzo, C.
N1 - Publisher Copyright:
© 2021, Universidad Autonoma Metropolitana. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Salmonella is one of the main pathogenic microorganisms present in foods, where natural orange juice is an example, although there are techniques that determine this bacterium, it is necessary to develop a device that allows Salmonella detection quickly, easily, and in real time. Therefore, using biosensors is an excellent option to solve this need. In this work, an optical biosensor of amorphous silicon thin films, a little studied material in this area, was applied for the detection of Salmonella Typhimurium in natural orange juice, using Fourier transform infrared spectroscopy. The characteristic detection infrared band was identified at 1030 cm-1, related to the functional groups presence of the cell membrane and DNA bacterial, Salmonella concentrations in a range from 100 to 1000 CFU/mL were detected and a SEM analysis was carried out. Biosensor did not show cross reactivity with enteropathogenic Escherichia coli.
AB - Salmonella is one of the main pathogenic microorganisms present in foods, where natural orange juice is an example, although there are techniques that determine this bacterium, it is necessary to develop a device that allows Salmonella detection quickly, easily, and in real time. Therefore, using biosensors is an excellent option to solve this need. In this work, an optical biosensor of amorphous silicon thin films, a little studied material in this area, was applied for the detection of Salmonella Typhimurium in natural orange juice, using Fourier transform infrared spectroscopy. The characteristic detection infrared band was identified at 1030 cm-1, related to the functional groups presence of the cell membrane and DNA bacterial, Salmonella concentrations in a range from 100 to 1000 CFU/mL were detected and a SEM analysis was carried out. Biosensor did not show cross reactivity with enteropathogenic Escherichia coli.
KW - Amorphous silicon
KW - Biosensor
KW - FTIR
KW - Orange juice
KW - Salmonella
UR - http://www.scopus.com/inward/record.url?scp=85119854398&partnerID=8YFLogxK
U2 - 10.24275/rmiq/Bio2538
DO - 10.24275/rmiq/Bio2538
M3 - Artículo
AN - SCOPUS:85119854398
SN - 1665-2738
VL - 20
JO - Revista Mexicana de Ingeniera Quimica
JF - Revista Mexicana de Ingeniera Quimica
IS - 3
M1 - Bio2538
ER -