Biofunctionalization process of a-SiC:H surfaces applied to an interdigitated microelectrode array to detect enterotoxigenic Escherichia coli

José Herrera-Celis, Claudia Reyes-Betanzo, Abdu Orduña-Díaz, Ana Pérez-Coyotl, Armando Hernández-Flores, Janet Morales-Chávez, Arely Culebro-Gomez

Resultado de la investigación: Capítulo del libro/informe/acta de congresoContribución a la conferenciarevisión exhaustiva

2 Citas (Scopus)

Resumen

A biofunctionalization process of a-SiC:H surfaces has been applied to an interdigitated microelectrode array (IMA) whose microelectrodes are covered by this material. The biofunctionalization process has been monitored stage by stage using Fourier transform infrared spectroscopy (FTIR), while its effects on the electrical behavior of the IMA were recorded in electrical impedance spectra. The process involves hydroxylation, silanization, generation of aldehyde groups, binding via protein A, immobilization of anti-Escherichia coli polyclonal antibodies, entrapping and detection of enterotoxigenic Escherichia coli (ETEC) in Luria-Bertani medium. The FTIR spectra confirm the success of the process. Regarding the performance of the IMA, although the detection of ETEC is successful and its percentage change in impedance reaches a value of 133.37% to 107 CFU/mL, some considerations may be taken into account to improve the sensitivity of the IMA by mean of the optimization of both the IMA design and the biofunctionalization process.

Idioma originalInglés
Título de la publicación alojada7th Latin American Congress on Biomedical Engineering, CLAIB 2016
EditoresJohn Bustamante, Daniel A. Sierra, Isnardo Torres
EditorialSpringer Verlag
Páginas34-37
Número de páginas4
ISBN (versión impresa)9789811040856
DOI
EstadoPublicada - 2017
Evento7th Latin American Congress on Biomedical Engineering, CLAIB 2016 - Bucaramanga, Santander, Colombia
Duración: 26 oct 201628 oct 2016

Serie de la publicación

NombreIFMBE Proceedings
Volumen60
ISSN (versión impresa)1680-0737

Conferencia

Conferencia7th Latin American Congress on Biomedical Engineering, CLAIB 2016
País/TerritorioColombia
CiudadBucaramanga, Santander
Período26/10/1628/10/16

Huella

Profundice en los temas de investigación de 'Biofunctionalization process of a-SiC:H surfaces applied to an interdigitated microelectrode array to detect enterotoxigenic Escherichia coli'. En conjunto forman una huella única.

Citar esto