Biofunctionalization process of a-SiC:H surfaces applied to an interdigitated microelectrode array to detect enterotoxigenic Escherichia coli

José Herrera-Celis; Claudia Reyes-Betanzo; Abdu Orduña-Díaz; Ana Pérez-Coyotl; Armando Hernández-Flores; Janet Morales-Chávez; Arely Culebro-Gomez

doi:10.1007/978-981-10-4086-3_9

Biofunctionalization process of a-SiC:H surfaces applied to an interdigitated microelectrode array to detect enterotoxigenic Escherichia coli

José Herrera-Celis, Claudia Reyes-Betanzo, Abdu Orduña-Díaz, Ana Pérez-Coyotl, Armando Hernández-Flores, Janet Morales-Chávez, Arely Culebro-Gomez

Centro de Investigación en Biotecnología Aplicada (CIBA), Unidad Tlaxcala

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

A biofunctionalization process of a-SiC:H surfaces has been applied to an interdigitated microelectrode array (IMA) whose microelectrodes are covered by this material. The biofunctionalization process has been monitored stage by stage using Fourier transform infrared spectroscopy (FTIR), while its effects on the electrical behavior of the IMA were recorded in electrical impedance spectra. The process involves hydroxylation, silanization, generation of aldehyde groups, binding via protein A, immobilization of anti-Escherichia coli polyclonal antibodies, entrapping and detection of enterotoxigenic Escherichia coli (ETEC) in Luria-Bertani medium. The FTIR spectra confirm the success of the process. Regarding the performance of the IMA, although the detection of ETEC is successful and its percentage change in impedance reaches a value of 133.37% to 107 CFU/mL, some considerations may be taken into account to improve the sensitivity of the IMA by mean of the optimization of both the IMA design and the biofunctionalization process.

Original language	English
Title of host publication	7th Latin American Congress on Biomedical Engineering, CLAIB 2016
Editors	John Bustamante, Daniel A. Sierra, Isnardo Torres
Publisher	Springer Verlag
Pages	34-37
Number of pages	4
ISBN (Print)	9789811040856
DOIs	https://doi.org/10.1007/978-981-10-4086-3_9
State	Published - 2017
Event	7th Latin American Congress on Biomedical Engineering, CLAIB 2016 - Bucaramanga, Santander, Colombia Duration: 26 Oct 2016 → 28 Oct 2016

Publication series

Name	IFMBE Proceedings
Volume	60
ISSN (Print)	1680-0737

Conference

Conference	7th Latin American Congress on Biomedical Engineering, CLAIB 2016
Country/Territory	Colombia
City	Bucaramanga, Santander
Period	26/10/16 → 28/10/16

Keywords

Biofunctionalization process
Electrical impedance spectroscopy
Enterotoxigenic Escherichia coli
Fourier transform infrared spectroscopy
Hydrogenated amorphous silicon carbide

Access to Document

10.1007/978-981-10-4086-3_9

Cite this

Herrera-Celis, J., Reyes-Betanzo, C., Orduña-Díaz, A., Pérez-Coyotl, A., Hernández-Flores, A., Morales-Chávez, J., & Culebro-Gomez, A. (2017). Biofunctionalization process of a-SiC:H surfaces applied to an interdigitated microelectrode array to detect enterotoxigenic Escherichia coli. In J. Bustamante, D. A. Sierra, & I. Torres (Eds.), 7th Latin American Congress on Biomedical Engineering, CLAIB 2016 (pp. 34-37). (IFMBE Proceedings; Vol. 60). Springer Verlag. https://doi.org/10.1007/978-981-10-4086-3_9

Herrera-Celis, José ; Reyes-Betanzo, Claudia ; Orduña-Díaz, Abdu et al. / Biofunctionalization process of a-SiC:H surfaces applied to an interdigitated microelectrode array to detect enterotoxigenic Escherichia coli. 7th Latin American Congress on Biomedical Engineering, CLAIB 2016. editor / John Bustamante ; Daniel A. Sierra ; Isnardo Torres. Springer Verlag, 2017. pp. 34-37 (IFMBE Proceedings).

@inproceedings{c8bb58f2019f4af180e0e657b1c75616,

title = "Biofunctionalization process of a-SiC:H surfaces applied to an interdigitated microelectrode array to detect enterotoxigenic Escherichia coli",

abstract = "A biofunctionalization process of a-SiC:H surfaces has been applied to an interdigitated microelectrode array (IMA) whose microelectrodes are covered by this material. The biofunctionalization process has been monitored stage by stage using Fourier transform infrared spectroscopy (FTIR), while its effects on the electrical behavior of the IMA were recorded in electrical impedance spectra. The process involves hydroxylation, silanization, generation of aldehyde groups, binding via protein A, immobilization of anti-Escherichia coli polyclonal antibodies, entrapping and detection of enterotoxigenic Escherichia coli (ETEC) in Luria-Bertani medium. The FTIR spectra confirm the success of the process. Regarding the performance of the IMA, although the detection of ETEC is successful and its percentage change in impedance reaches a value of 133.37% to 107 CFU/mL, some considerations may be taken into account to improve the sensitivity of the IMA by mean of the optimization of both the IMA design and the biofunctionalization process.",

keywords = "Biofunctionalization process, Electrical impedance spectroscopy, Enterotoxigenic Escherichia coli, Fourier transform infrared spectroscopy, Hydrogenated amorphous silicon carbide",

author = "Jos{\'e} Herrera-Celis and Claudia Reyes-Betanzo and Abdu Ordu{\~n}a-D{\'i}az and Ana P{\'e}rez-Coyotl and Armando Hern{\'a}ndez-Flores and Janet Morales-Ch{\'a}vez and Arely Culebro-Gomez",

note = "Publisher Copyright: {\textcopyright} Springer Nature Singapore Pte Ltd. 2017.; 7th Latin American Congress on Biomedical Engineering, CLAIB 2016 ; Conference date: 26-10-2016 Through 28-10-2016",

year = "2017",

doi = "10.1007/978-981-10-4086-3_9",

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isbn = "9789811040856",

series = "IFMBE Proceedings",

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Herrera-Celis, J, Reyes-Betanzo, C, Orduña-Díaz, A, Pérez-Coyotl, A, Hernández-Flores, A, Morales-Chávez, J & Culebro-Gomez, A 2017, Biofunctionalization process of a-SiC:H surfaces applied to an interdigitated microelectrode array to detect enterotoxigenic Escherichia coli. in J Bustamante, DA Sierra & I Torres (eds), 7th Latin American Congress on Biomedical Engineering, CLAIB 2016. IFMBE Proceedings, vol. 60, Springer Verlag, pp. 34-37, 7th Latin American Congress on Biomedical Engineering, CLAIB 2016, Bucaramanga, Santander, Colombia, 26/10/16. https://doi.org/10.1007/978-981-10-4086-3_9

Biofunctionalization process of a-SiC:H surfaces applied to an interdigitated microelectrode array to detect enterotoxigenic Escherichia coli. / Herrera-Celis, José; Reyes-Betanzo, Claudia; Orduña-Díaz, Abdu et al.
7th Latin American Congress on Biomedical Engineering, CLAIB 2016. ed. / John Bustamante; Daniel A. Sierra; Isnardo Torres. Springer Verlag, 2017. p. 34-37 (IFMBE Proceedings; Vol. 60).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Biofunctionalization process of a-SiC:H surfaces applied to an interdigitated microelectrode array to detect enterotoxigenic Escherichia coli

AU - Herrera-Celis, José

AU - Reyes-Betanzo, Claudia

AU - Orduña-Díaz, Abdu

AU - Pérez-Coyotl, Ana

AU - Hernández-Flores, Armando

AU - Morales-Chávez, Janet

AU - Culebro-Gomez, Arely

N1 - Publisher Copyright: © Springer Nature Singapore Pte Ltd. 2017.

PY - 2017

Y1 - 2017

N2 - A biofunctionalization process of a-SiC:H surfaces has been applied to an interdigitated microelectrode array (IMA) whose microelectrodes are covered by this material. The biofunctionalization process has been monitored stage by stage using Fourier transform infrared spectroscopy (FTIR), while its effects on the electrical behavior of the IMA were recorded in electrical impedance spectra. The process involves hydroxylation, silanization, generation of aldehyde groups, binding via protein A, immobilization of anti-Escherichia coli polyclonal antibodies, entrapping and detection of enterotoxigenic Escherichia coli (ETEC) in Luria-Bertani medium. The FTIR spectra confirm the success of the process. Regarding the performance of the IMA, although the detection of ETEC is successful and its percentage change in impedance reaches a value of 133.37% to 107 CFU/mL, some considerations may be taken into account to improve the sensitivity of the IMA by mean of the optimization of both the IMA design and the biofunctionalization process.

AB - A biofunctionalization process of a-SiC:H surfaces has been applied to an interdigitated microelectrode array (IMA) whose microelectrodes are covered by this material. The biofunctionalization process has been monitored stage by stage using Fourier transform infrared spectroscopy (FTIR), while its effects on the electrical behavior of the IMA were recorded in electrical impedance spectra. The process involves hydroxylation, silanization, generation of aldehyde groups, binding via protein A, immobilization of anti-Escherichia coli polyclonal antibodies, entrapping and detection of enterotoxigenic Escherichia coli (ETEC) in Luria-Bertani medium. The FTIR spectra confirm the success of the process. Regarding the performance of the IMA, although the detection of ETEC is successful and its percentage change in impedance reaches a value of 133.37% to 107 CFU/mL, some considerations may be taken into account to improve the sensitivity of the IMA by mean of the optimization of both the IMA design and the biofunctionalization process.

KW - Biofunctionalization process

KW - Electrical impedance spectroscopy

KW - Enterotoxigenic Escherichia coli

KW - Fourier transform infrared spectroscopy

KW - Hydrogenated amorphous silicon carbide

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U2 - 10.1007/978-981-10-4086-3_9

DO - 10.1007/978-981-10-4086-3_9

M3 - Contribución a la conferencia

AN - SCOPUS:85018407882

SN - 9789811040856

T3 - IFMBE Proceedings

SP - 34

EP - 37

BT - 7th Latin American Congress on Biomedical Engineering, CLAIB 2016

A2 - Bustamante, John

A2 - Sierra, Daniel A.

A2 - Torres, Isnardo

PB - Springer Verlag

T2 - 7th Latin American Congress on Biomedical Engineering, CLAIB 2016

Y2 - 26 October 2016 through 28 October 2016

ER -

Herrera-Celis J, Reyes-Betanzo C, Orduña-Díaz A, Pérez-Coyotl A, Hernández-Flores A, Morales-Chávez J et al. Biofunctionalization process of a-SiC:H surfaces applied to an interdigitated microelectrode array to detect enterotoxigenic Escherichia coli. In Bustamante J, Sierra DA, Torres I, editors, 7th Latin American Congress on Biomedical Engineering, CLAIB 2016. Springer Verlag. 2017. p. 34-37. (IFMBE Proceedings). doi: 10.1007/978-981-10-4086-3_9

Biofunctionalization process of a-SiC:H surfaces applied to an interdigitated microelectrode array to detect enterotoxigenic Escherichia coli

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