TY - JOUR
T1 - Bacillus licheniformis BCR 4-3 increases immune response and survival of Litopenaeus vannamei challenged with Vibrio parahaemolyticus IPNGS16
AU - García-Medel, Darío Israel
AU - Angulo, Carlos
AU - Escamilla-Montes, Ruth
AU - Fierro-Coronado, Jesús Arturo
AU - Diarte-Plata, Genaro
AU - Gámez-Jiménez, Carina
AU - Luna-González, Antonio
N1 - Publisher Copyright:
© 2020, Springer Nature Switzerland AG.
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Immunoprophylactic alternatives are needed to fight against acute hepatopancreatic necrosis disease (AHPND). This study addressed the effects of different routes of Bacillus administration (via diet or culture water) on growth performance, survival, and immune parameters of Litopenaeus vannamei challenged with Vibrio parahaemolyticus-AHPND. Bacillus mixture and Bacillus licheniformis BCR 4-3 were added to feed (1 × 106 CFU g−1) or rearing water (1 × 106, 2 × 106, 3 × 106 CFU L−1). Animals were challenged with a determined median lethal V. parahaemolyticus concentration. Survival, growth performance, and immune response were determined. The results showed that B. licheniformis BCR 4-3 (3 × 106 CFU L−1) administration via culture water significantly (P < 0.05) increased survival (53.4%) in shrimp challenged with V. parahaemolyticus; growth performance was also significantly (P < 0.05) higher compared to the control group. Moreover, the relative expression of innate immune-related genes, such as lysozyme, penaeidin4, crustin, and superoxide dismutase, significantly (P < 0.05) upregulated in shrimp fed B. licheniformis BCR4-3 compared with the control group. In conclusion, the administration of probiotic B. licheniformis BCR4-3 in feed and culture water increased resistance in L. vannamei against V. parahaemolyticus-AHPND and was found associated with an upregulation of key antimicrobial innate immune-related genes.
AB - Immunoprophylactic alternatives are needed to fight against acute hepatopancreatic necrosis disease (AHPND). This study addressed the effects of different routes of Bacillus administration (via diet or culture water) on growth performance, survival, and immune parameters of Litopenaeus vannamei challenged with Vibrio parahaemolyticus-AHPND. Bacillus mixture and Bacillus licheniformis BCR 4-3 were added to feed (1 × 106 CFU g−1) or rearing water (1 × 106, 2 × 106, 3 × 106 CFU L−1). Animals were challenged with a determined median lethal V. parahaemolyticus concentration. Survival, growth performance, and immune response were determined. The results showed that B. licheniformis BCR 4-3 (3 × 106 CFU L−1) administration via culture water significantly (P < 0.05) increased survival (53.4%) in shrimp challenged with V. parahaemolyticus; growth performance was also significantly (P < 0.05) higher compared to the control group. Moreover, the relative expression of innate immune-related genes, such as lysozyme, penaeidin4, crustin, and superoxide dismutase, significantly (P < 0.05) upregulated in shrimp fed B. licheniformis BCR4-3 compared with the control group. In conclusion, the administration of probiotic B. licheniformis BCR4-3 in feed and culture water increased resistance in L. vannamei against V. parahaemolyticus-AHPND and was found associated with an upregulation of key antimicrobial innate immune-related genes.
KW - Antimicrobial peptides
KW - Bacillus
KW - Immune response
KW - Shrimp
KW - Vibrio
UR - http://www.scopus.com/inward/record.url?scp=85089358197&partnerID=8YFLogxK
U2 - 10.1007/s10499-020-00585-2
DO - 10.1007/s10499-020-00585-2
M3 - Artículo
AN - SCOPUS:85089358197
SN - 0967-6120
VL - 28
SP - 2303
EP - 2318
JO - Aquaculture International
JF - Aquaculture International
IS - 6
ER -