TY - JOUR
T1 - Antimutagenic properties of affinin isolated from Heliopsis longipes extract
AU - Arriaga-Alba, Myriam
AU - Rios, María Yolanda
AU - Déciga-Campos, Myrna
N1 - Funding Information:
1Laboratorio de Investigación en Microbiología, Dirección de Investigación y Enseñanza, Hospital Juárez de México, Av. Instituto Politécnico Nacional, 5160 México D.F., México, 2Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Cuernavaca, Morelos, México, and 3Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico, Nacional Plan de San Luis y Díaz Mirón Col., Santo Tomás, México, D.F, México
PY - 2013
Y1 - 2013
N2 - Context: Heliopsis longipes (A. Gray) Blake (Asteraceae), commonly known in Mexico as "chilcuage" or "chilcuan", is widely used as an analgesic and anesthetic agent. Affinin, the major metabolite of this plant, and the ethanol extract of the plant have shown antinociceptive properties in mice. H. longipes plant produces a complex mixture of antioxidant chlorophylls and polyamines as well as a number of possible antimutagens. Objective: The current study evaluated the potential utilization of the natural product affinin isolated from H. longipes ethanol extract as an antimutagenic and possibly anticarcinogenic agent. Materials and methods: The Ames assay was used to assess the mutagenic properties of affinin (12.5, 25 and 50 mg/plate) that was added to several mutagens with or without S9 metabolic activation in Salmonella typhimurium (TA98, TA100 and TA102 strains). Results: Heliopsis longipes extract and affinin were not toxic as a reduction in the number of His revertant bacteria colonies. Affinin (25 and 50 mg/plate) significantly reduced the frameshift mutations that were generated by 2-aminoanthracene (2AA) (40%) and reduced the oxidative DNA damage generated by norfloxacin (NOR) (37-50%). Affinin possessed antioxidant properties that were able to reduce 2AA- and NOR-induced mutations in S. typhimurium TA98 and TA102, respectively. Discussion and conclusion: Affinin, the principal metabolite of H. longipes, is not mutagenic and possesses antimutagenic activity. These plants are currently used to treat some pain symptoms in Mexico; and antimutagen activity determined could be important to treat some pain symptoms related to antiradical activity.
AB - Context: Heliopsis longipes (A. Gray) Blake (Asteraceae), commonly known in Mexico as "chilcuage" or "chilcuan", is widely used as an analgesic and anesthetic agent. Affinin, the major metabolite of this plant, and the ethanol extract of the plant have shown antinociceptive properties in mice. H. longipes plant produces a complex mixture of antioxidant chlorophylls and polyamines as well as a number of possible antimutagens. Objective: The current study evaluated the potential utilization of the natural product affinin isolated from H. longipes ethanol extract as an antimutagenic and possibly anticarcinogenic agent. Materials and methods: The Ames assay was used to assess the mutagenic properties of affinin (12.5, 25 and 50 mg/plate) that was added to several mutagens with or without S9 metabolic activation in Salmonella typhimurium (TA98, TA100 and TA102 strains). Results: Heliopsis longipes extract and affinin were not toxic as a reduction in the number of His revertant bacteria colonies. Affinin (25 and 50 mg/plate) significantly reduced the frameshift mutations that were generated by 2-aminoanthracene (2AA) (40%) and reduced the oxidative DNA damage generated by norfloxacin (NOR) (37-50%). Affinin possessed antioxidant properties that were able to reduce 2AA- and NOR-induced mutations in S. typhimurium TA98 and TA102, respectively. Discussion and conclusion: Affinin, the principal metabolite of H. longipes, is not mutagenic and possesses antimutagenic activity. These plants are currently used to treat some pain symptoms in Mexico; and antimutagen activity determined could be important to treat some pain symptoms related to antiradical activity.
KW - Ames test
KW - Analgesic
KW - Antinociceptive
KW - Asteraceae
KW - Mexican plant
UR - http://www.scopus.com/inward/record.url?scp=84885398159&partnerID=8YFLogxK
U2 - 10.3109/13880209.2013.775161
DO - 10.3109/13880209.2013.775161
M3 - Artículo
C2 - 23738801
SN - 1388-0209
VL - 51
SP - 1035
EP - 1039
JO - Pharmaceutical Biology
JF - Pharmaceutical Biology
IS - 8
ER -