TY - JOUR
T1 - An overview of the infection of CMV, HSV 1/2 and EBV in Mexican patients with glioblastoma multiforme
AU - Zavala-Vega, Sergio
AU - Castro-Escarpulli, Graciela
AU - Hernández-Santos, Hector
AU - Salinas-Lara, Citlatepetl
AU - Palma, Icela
AU - Mejía-Aranguré, Juan Manuel
AU - Gelista-Herrera, Noemí
AU - Rembao-Bojorquez, Daniel
AU - Ochoa, Sara A.
AU - Cruz-Córdova, Ariadnna
AU - Xicohtencatl-Cortes, Juan
AU - Uribe-Gutiérrez, Gabriel
AU - Arellano-Galindo, José
N1 - Publisher Copyright:
© 2017 Elsevier GmbH
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Several risk factors are involved in glioblastoma, including cytomegalovirus (CMV). This research was carried out to determine the rate of CMV infection, as well as HSV 1/2 and EBV in brain tissue, in patients with glioblastomamultiforme (GBM). The tissues were tested using immunohistochemistry, PCR, in situ hybridization and real-time PCR. At least, one HHV was detected in 21/29 (72%) patients as follows: single infections with HSV-1/2 in 4/21 (19%), EBV in 6/21 (28.6%) and CMV in 1/21 (4.8%). Mixed viral infection, HSV-1/2 and EBV were detected in 4/21 patients (19%), CMV and EBV in 5/21 (23.8%), and HSV-1/2, EBV, and CMV in 1/21. The CMV viral load ranged from 3 × 102 to 4.33 × 105 genome/100 ng of tissue. Genotype based on CMV gB was 3/7 where 2/3 was gB1 and 1/3 gB4. HSV, EBV and CMV were frequently found in brain tissues, more in mix in a population reported as highly seropositive.
AB - Several risk factors are involved in glioblastoma, including cytomegalovirus (CMV). This research was carried out to determine the rate of CMV infection, as well as HSV 1/2 and EBV in brain tissue, in patients with glioblastomamultiforme (GBM). The tissues were tested using immunohistochemistry, PCR, in situ hybridization and real-time PCR. At least, one HHV was detected in 21/29 (72%) patients as follows: single infections with HSV-1/2 in 4/21 (19%), EBV in 6/21 (28.6%) and CMV in 1/21 (4.8%). Mixed viral infection, HSV-1/2 and EBV were detected in 4/21 patients (19%), CMV and EBV in 5/21 (23.8%), and HSV-1/2, EBV, and CMV in 1/21. The CMV viral load ranged from 3 × 102 to 4.33 × 105 genome/100 ng of tissue. Genotype based on CMV gB was 3/7 where 2/3 was gB1 and 1/3 gB4. HSV, EBV and CMV were frequently found in brain tissues, more in mix in a population reported as highly seropositive.
KW - Cytomegalovirus
KW - Epstein–Barr
KW - Glioblastomamultiforme
KW - HHVs
KW - Herpes
KW - Viral infection
UR - http://www.scopus.com/inward/record.url?scp=85012904970&partnerID=8YFLogxK
U2 - 10.1016/j.prp.2016.12.006
DO - 10.1016/j.prp.2016.12.006
M3 - Artículo
C2 - 28215646
SN - 0344-0338
VL - 213
SP - 271
EP - 276
JO - Pathology Research and Practice
JF - Pathology Research and Practice
IS - 3
ER -