The sigma factor SigD of Mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments

Miguel A. Ares; Nora Rios-Sarabia; Miguel A. De La Cruz; Sandra Rivera-Gutierrez; Lazaro Garcia-Morales; Lizbel Leon-Solis; Clara Espitia; Sabino Pacheco; Jorge F. Cerna-Cortes; A. Cecilia Helguera-Repetto; Maria Jesus Garcia; Jorge A. Gonzalez-y-Merchand

The sigma factor SigD of Mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments

Miguel A. Ares, Nora Rios-Sarabia, Miguel A. De La Cruz, Sandra Rivera-Gutierrez, Lazaro Garcia-Morales, Lizbel Leon-Solis, Clara Espitia, Sabino Pacheco, Jorge F. Cerna-Cortes, A. Cecilia Helguera-Repetto, Maria Jesus Garcia, Jorge A. Gonzalez-y-Merchand

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

This work examined the expression of the septum site determining gene (ssd) of Mycobacterium tuberculosis CDC1551 and its ΔsigD mutant under different growing conditions. The results showed an up-regulation of ssd during stationary phase and starvation conditions, but not during in vitro dormancy, suggesting a putative role for SigD in the control of ssd expression mainly under lack-of-nutrients environments. Furthermore, we elucidated a putative link between ssd expression and cell elongation of bacilli at stationary phase. In addition, a -35 sigD consensus sequence was found for the ssd promoter region, reinforcing the putative regulation of ssd by SigD, and in turn, supporting this protein role during the adaptation of M. tuberculosis to some stressful environments.

Original language	English
Pages (from-to)	199-204
Number of pages	6
Journal	New Microbiologica
Volume	40
Issue number	3
State	Published - Jul 2017

Keywords

M. tuberculosis
Septum site determining protein
SigD
Stressful environments
Type IV pili

Cite this

Ares, M. A., Rios-Sarabia, N., De La Cruz, M. A., Rivera-Gutierrez, S., Garcia-Morales, L., Leon-Solis, L., Espitia, C., Pacheco, S., Cerna-Cortes, J. F., Helguera-Repetto, A. C., Garcia, M. J., & Gonzalez-y-Merchand, J. A. (2017). The sigma factor SigD of Mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments. New Microbiologica, 40(3), 199-204.

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title = "The sigma factor SigD of Mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments",

abstract = "This work examined the expression of the septum site determining gene (ssd) of Mycobacterium tuberculosis CDC1551 and its ΔsigD mutant under different growing conditions. The results showed an up-regulation of ssd during stationary phase and starvation conditions, but not during in vitro dormancy, suggesting a putative role for SigD in the control of ssd expression mainly under lack-of-nutrients environments. Furthermore, we elucidated a putative link between ssd expression and cell elongation of bacilli at stationary phase. In addition, a -35 sigD consensus sequence was found for the ssd promoter region, reinforcing the putative regulation of ssd by SigD, and in turn, supporting this protein role during the adaptation of M. tuberculosis to some stressful environments.",

keywords = "M. tuberculosis, Septum site determining protein, SigD, Stressful environments, Type IV pili",

author = "Ares, {Miguel A.} and Nora Rios-Sarabia and {De La Cruz}, {Miguel A.} and Sandra Rivera-Gutierrez and Lazaro Garcia-Morales and Lizbel Leon-Solis and Clara Espitia and Sabino Pacheco and Cerna-Cortes, {Jorge F.} and Helguera-Repetto, {A. Cecilia} and Garcia, {Maria Jesus} and Gonzalez-y-Merchand, {Jorge A.}",

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Ares, MA, Rios-Sarabia, N, De La Cruz, MA, Rivera-Gutierrez, S, Garcia-Morales, L, Leon-Solis, L, Espitia, C, Pacheco, S, Cerna-Cortes, JF, Helguera-Repetto, AC, Garcia, MJ & Gonzalez-y-Merchand, JA 2017, 'The sigma factor SigD of Mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments', New Microbiologica, vol. 40, no. 3, pp. 199-204.

TY - JOUR

T1 - The sigma factor SigD of Mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments

AU - Ares, Miguel A.

AU - Rios-Sarabia, Nora

AU - De La Cruz, Miguel A.

AU - Rivera-Gutierrez, Sandra

AU - Garcia-Morales, Lazaro

AU - Leon-Solis, Lizbel

AU - Espitia, Clara

AU - Pacheco, Sabino

AU - Cerna-Cortes, Jorge F.

AU - Helguera-Repetto, A. Cecilia

AU - Garcia, Maria Jesus

AU - Gonzalez-y-Merchand, Jorge A.

PY - 2017/7

Y1 - 2017/7

N2 - This work examined the expression of the septum site determining gene (ssd) of Mycobacterium tuberculosis CDC1551 and its ΔsigD mutant under different growing conditions. The results showed an up-regulation of ssd during stationary phase and starvation conditions, but not during in vitro dormancy, suggesting a putative role for SigD in the control of ssd expression mainly under lack-of-nutrients environments. Furthermore, we elucidated a putative link between ssd expression and cell elongation of bacilli at stationary phase. In addition, a -35 sigD consensus sequence was found for the ssd promoter region, reinforcing the putative regulation of ssd by SigD, and in turn, supporting this protein role during the adaptation of M. tuberculosis to some stressful environments.

AB - This work examined the expression of the septum site determining gene (ssd) of Mycobacterium tuberculosis CDC1551 and its ΔsigD mutant under different growing conditions. The results showed an up-regulation of ssd during stationary phase and starvation conditions, but not during in vitro dormancy, suggesting a putative role for SigD in the control of ssd expression mainly under lack-of-nutrients environments. Furthermore, we elucidated a putative link between ssd expression and cell elongation of bacilli at stationary phase. In addition, a -35 sigD consensus sequence was found for the ssd promoter region, reinforcing the putative regulation of ssd by SigD, and in turn, supporting this protein role during the adaptation of M. tuberculosis to some stressful environments.

KW - M. tuberculosis

KW - Septum site determining protein

KW - SigD

KW - Stressful environments

KW - Type IV pili

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M3 - Artículo

SN - 1121-7138

VL - 40

SP - 199

EP - 204

JO - New Microbiologica

JF - New Microbiologica

IS - 3

ER -

The sigma factor SigD of Mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments

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