The mitochondrial respiratory chain of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill

Leobarda Robles-Martínez, María Guadalupe Guerra-Sánchez, Oscar Flores-Herrera, Ana Niurka Hernández-Lauzardo, Miguel Gerardo Velazquez-Del Valle, Juan Pablo Pardo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Rhizopus stolonifer (Ehrenb.:Fr.) Vuill mitochondria contain the complete system for oxidative phosphorylation, formed by the classical components of the electron transport chain (complexes I, II, III, and IV) and the F 1F0-ATP synthase (complex V). Using the native gel electrophoresis, we have shown the existence of supramolecular associations of the respiratory complexes. The composition and stoichiometry of the oxidative phosphorylation complexes were similar to those found in other organisms. Additionally, two alternative routes for the oxidation of cytosolic NADH were identified: the alternative NADH dehydrogenase and the glycerol-3-phosphate shuttles. Residual respiratory activity after inhibition of complex IV by cyanide was inhibited by low concentrations of n-octyl gallate, indicating the presence ofanalternative oxidase. The K0.5 for the respiratory substrates NADH, succinate, and glycerol-3-phosphate in permeabilized cells was higher than in isolated mitochondria, suggesting that interactions of mitochondria with other cellular elements might be important for the function of this organelle. © Springer-Verlag Berlin Heidelberg 2012.
Original languageAmerican English
Pages (from-to)51-61
Number of pages44
JournalArchives of Microbiology
DOIs
StatePublished - 1 Jan 2013

Fingerprint

Rhizopus
Mitochondria
Electron Transport
Oxidative Phosphorylation
NAD
Electron Transport Complex I
NADH Dehydrogenase
Cyanides
Succinic Acid
Berlin
Electrophoresis
Stoichiometry
Organelles
Oxidoreductases
Adenosine Triphosphate
Gels
Oxidation
Substrates
Chemical analysis
alpha-glycerophosphoric acid

Cite this

Robles-Martínez, Leobarda ; Guerra-Sánchez, María Guadalupe ; Flores-Herrera, Oscar ; Hernández-Lauzardo, Ana Niurka ; Velazquez-Del Valle, Miguel Gerardo ; Pardo, Juan Pablo. / The mitochondrial respiratory chain of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill. In: Archives of Microbiology. 2013 ; pp. 51-61.
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The mitochondrial respiratory chain of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill. / Robles-Martínez, Leobarda; Guerra-Sánchez, María Guadalupe; Flores-Herrera, Oscar; Hernández-Lauzardo, Ana Niurka; Velazquez-Del Valle, Miguel Gerardo; Pardo, Juan Pablo.

In: Archives of Microbiology, 01.01.2013, p. 51-61.

Research output: Contribution to journalArticle

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