The mitochondrial respiratory chain of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill

Leobarda Robles-Martínez, María Guadalupe Guerra-Sánchez, Oscar Flores-Herrera, Ana Niurka Hernández-Lauzardo, Miguel Gerardo Velázquez-Del Valle, Juan Pablo Pardo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

8 Citas (Scopus)

Resumen

Rhizopus stolonifer (Ehrenb.:Fr.) Vuill mitochondria contain the complete system for oxidative phosphorylation, formed by the classical components of the electron transport chain (complexes I, II, III, and IV) and the F 1 F 0 -ATP synthase (complex V). Using the native gel electrophoresis, we have shown the existence of supramolecular associations of the respiratory complexes. The composition and stoichiometry of the oxidative phosphorylation complexes were similar to those found in other organisms. Additionally, two alternative routes for the oxidation of cytosolic NADH were identified: the alternative NADH dehydrogenase and the glycerol-3-phosphate shuttles. Residual respiratory activity after inhibition of complex IV by cyanide was inhibited by low concentrations of n-octyl gallate, indicating the presence ofanalternative oxidase. The K 0 .5 for the respiratory substrates NADH, succinate, and glycerol-3-phosphate in permeabilized cells was higher than in isolated mitochondria, suggesting that interactions of mitochondria with other cellular elements might be important for the function of this organelle.

Idioma originalInglés
Páginas (desde-hasta)51-61
Número de páginas11
PublicaciónArchives of Microbiology
Volumen195
N.º1
DOI
EstadoPublicada - ene. 2013

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