TY - JOUR
T1 - Phytochemical profiling of coryphantha macromeris (cactaceae) growing in greenhouse conditions using ultra-high-performance liquid chromatography–tandem mass spectrometry
AU - Cabañas-García, Emmanuel
AU - Areche, Carlos
AU - Jáuregui-Rincón, Juan
AU - Cruz-Sosa, Francisco
AU - Balch, Eugenio Pérez Molphe
N1 - Publisher Copyright:
© 2019 by the authors.
PY - 2019/2/15
Y1 - 2019/2/15
N2 - Chromatographic separation combined with mass spectrometry is a powerful tool for the characterization of plant metabolites because of its high sensitivity and selectivity. In this work, the phytochemical profile of aerial and radicular parts of Coryphantha macromeris (Engelm.) Britton & Rose growing under greenhouse conditions was qualitatively investigated for the first time by means of modern ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-PDA-HESI-Orbitrap-MS/MS). The UHPLC-PDA-HESI-Orbitrap-MS/MS analysis indicated a high complexity in phenolic metabolites. In our investigation, 69 compounds were detected and 60 of them were identified. Among detected compounds, several phenolic acids, phenolic glycosides, and organic acids were found. Within this diversity, 26 metabolites were exclusively detected in the aerial part, and 19 in the roots. Twenty-four metabolites occurred in both plant parts. According to the relative abundance of peaks in the chromatogram, ferulic and piscidic acids and their derivatives may correspond to one of the main phenolic compounds of C. macromeris. Our results contribute to the phytochemical knowledge regarding C. macromeris and its potential applications in the pharmaceutical and cosmetic industries. Besides, some metabolites and their fragmentation patterns are reported here for the first time for cacti species.
AB - Chromatographic separation combined with mass spectrometry is a powerful tool for the characterization of plant metabolites because of its high sensitivity and selectivity. In this work, the phytochemical profile of aerial and radicular parts of Coryphantha macromeris (Engelm.) Britton & Rose growing under greenhouse conditions was qualitatively investigated for the first time by means of modern ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-PDA-HESI-Orbitrap-MS/MS). The UHPLC-PDA-HESI-Orbitrap-MS/MS analysis indicated a high complexity in phenolic metabolites. In our investigation, 69 compounds were detected and 60 of them were identified. Among detected compounds, several phenolic acids, phenolic glycosides, and organic acids were found. Within this diversity, 26 metabolites were exclusively detected in the aerial part, and 19 in the roots. Twenty-four metabolites occurred in both plant parts. According to the relative abundance of peaks in the chromatogram, ferulic and piscidic acids and their derivatives may correspond to one of the main phenolic compounds of C. macromeris. Our results contribute to the phytochemical knowledge regarding C. macromeris and its potential applications in the pharmaceutical and cosmetic industries. Besides, some metabolites and their fragmentation patterns are reported here for the first time for cacti species.
KW - Active compounds
KW - Cactaceae
KW - Fragmentation pattern
KW - Phenolic compounds
KW - Secondary metabolites
KW - Succulent plants
KW - UHPLC
UR - http://www.scopus.com/inward/record.url?scp=85061628529&partnerID=8YFLogxK
U2 - 10.3390/molecules24040705
DO - 10.3390/molecules24040705
M3 - Artículo
C2 - 30781375
AN - SCOPUS:85061628529
SN - 1420-3049
VL - 24
JO - Molecules
JF - Molecules
IS - 4
M1 - 705
ER -