TY - JOUR
T1 - Partial characterization of bacteriocin produced by halotolerant Pediococcus acidilactici strain QC38 isolated from traditional Cotija cheese
AU - Morales-Estrada, Aurea I.
AU - López-Merino, Ahidé
AU - Gutierrez-Mendez, Nestor
AU - Ruiz, Enrico A.
AU - Contreras-Rodríguez, Araceli
N1 - Funding Information:
This work was funded by CONACYT CB-2011-01 No. 169259, SIP-PN 20131630, 20144471 and ICYT-DF/IPN. AIME was supported by CONACYT and PIFI-IPN scholarships. ALM, EAR, and ACR were supported by fellowships from COFAA-IPN, SIP-EDI, and SNI-CONACYT. ACR was supported by sabbatical semester 2013-2014 ENCB-IPN.
PY - 2016
Y1 - 2016
N2 - During a screening of lactic acid bacteria producing bacteriocin from Cotija cheese, the strain QC38 was isolated. Based on the 16S rRNA gene nucleotide sequencing (516 pb accession no KJ210322) and phylogenetic analysis, the isolate was identified as Pediococcus acidilactici. Neutralized cell-free supernatant was tested for antimicrobial activity against 17 Gram-negative and Gram-positive pathogens. Growth inhibition was achieved against Listeria monocytogenes (supplier or indication or source), Staphylococcus aureus, Vibrio vulnificus, Vibrio cholerae O1 Ogawa, Vibrio cholerae NO 01 and Salmonella enterica subsp. Enterica serovar Typhimurium. Bacteriocin-like substance, after heating at 121°C for 15 min it remained stable and its antimicrobial activity was observed at pH ranging from 1.0 to 10.0 but inactivated by α-chymotrypsin and proteinase K. Strain QC38 was able to grow in 1-9% NaCl concentration. The plate overlay assayshowed an approximate size of bacteriocin-like substance between 3.4 and 6.5 kDa. P. acidilactici QC38 harboured a plasmid that contains a gene for a pediocin (PA-1).
AB - During a screening of lactic acid bacteria producing bacteriocin from Cotija cheese, the strain QC38 was isolated. Based on the 16S rRNA gene nucleotide sequencing (516 pb accession no KJ210322) and phylogenetic analysis, the isolate was identified as Pediococcus acidilactici. Neutralized cell-free supernatant was tested for antimicrobial activity against 17 Gram-negative and Gram-positive pathogens. Growth inhibition was achieved against Listeria monocytogenes (supplier or indication or source), Staphylococcus aureus, Vibrio vulnificus, Vibrio cholerae O1 Ogawa, Vibrio cholerae NO 01 and Salmonella enterica subsp. Enterica serovar Typhimurium. Bacteriocin-like substance, after heating at 121°C for 15 min it remained stable and its antimicrobial activity was observed at pH ranging from 1.0 to 10.0 but inactivated by α-chymotrypsin and proteinase K. Strain QC38 was able to grow in 1-9% NaCl concentration. The plate overlay assayshowed an approximate size of bacteriocin-like substance between 3.4 and 6.5 kDa. P. acidilactici QC38 harboured a plasmid that contains a gene for a pediocin (PA-1).
KW - Bacteriocin
KW - Cheese
KW - Cotija
KW - Lactic acid bacteria
KW - Pediococcus acidilactici
UR - http://www.scopus.com/inward/record.url?scp=84991082167&partnerID=8YFLogxK
U2 - 10.5604/17331331.1215607
DO - 10.5604/17331331.1215607
M3 - Artículo
C2 - 29334047
SN - 1733-1331
VL - 65
SP - 279
EP - 285
JO - Polish Journal of Microbiology
JF - Polish Journal of Microbiology
IS - 3
ER -