TY - JOUR
T1 - Methods of micropatterning and manipulation of cells for biomedical applications
AU - Martinez-Rivas, Adrian
AU - González-Quijano, Génesis K.
AU - Proa-Coronado, Sergio
AU - Séverac, Childérick
AU - Dague, Etienne
N1 - Publisher Copyright:
© 2017 by the authors.
PY - 2017/11/29
Y1 - 2017/11/29
N2 - Micropatterning and manipulation of mammalian and bacterial cells are important in biomedical studies to perform in vitro assays and to evaluate biochemical processes accurately, establishing the basis for implementing biomedical microelectromechanical systems (bioMEMS), point-of-care (POC) devices, or organs-on-chips (OOC), which impact on neurological, oncological, dermatologic, or tissue engineering issues as part of personalizedmedicine. Cell patterning represents a crucial step in fundamental and applied biological studies in vitro, hence today there are a myriad of materials and techniques that allow one to immobilize and manipulate cells, imitating the 3D in vivo milieu. This review focuses on current physical cell patterning, plus chemical and a combination of them both that utilizes different materials and cutting-edge micro-nanofabrication methodologies.
AB - Micropatterning and manipulation of mammalian and bacterial cells are important in biomedical studies to perform in vitro assays and to evaluate biochemical processes accurately, establishing the basis for implementing biomedical microelectromechanical systems (bioMEMS), point-of-care (POC) devices, or organs-on-chips (OOC), which impact on neurological, oncological, dermatologic, or tissue engineering issues as part of personalizedmedicine. Cell patterning represents a crucial step in fundamental and applied biological studies in vitro, hence today there are a myriad of materials and techniques that allow one to immobilize and manipulate cells, imitating the 3D in vivo milieu. This review focuses on current physical cell patterning, plus chemical and a combination of them both that utilizes different materials and cutting-edge micro-nanofabrication methodologies.
KW - Biomedical microelectromechanical systems (bioMEMS)
KW - Cell patterning and manipulation
KW - Mammalian and bacterial cells
KW - Micro-nanofabrication
KW - Microfluidics
KW - Organs-on-chips (OOC)
KW - Point-of-care (POC)
KW - Soft lithography
UR - http://www.scopus.com/inward/record.url?scp=85036458480&partnerID=8YFLogxK
U2 - 10.3390/mi8120347
DO - 10.3390/mi8120347
M3 - Artículo de revisión
SN - 2072-666X
VL - 8
JO - Micromachines
JF - Micromachines
IS - 12
M1 - 347
ER -