JVG9, A benzimidazole derivative, Alters the surface and cytoskeleton of Trypanosoma cruzi bloodstream trypomastigotes

Dylan L. Díaz-Chiguer, Francisco Hernández-Luis, BenjamíN Nogueda-Torres, Rafael Castillo, Olivia Reynoso-Ducoing, Alicia Hernández-Campos, Javier R. Ambrosio

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

© 2014 Fundacao Oswaldo Cruz. All rights reserved. Trypanosoma cruzi has a particular cytoskeleton that consists of a subpellicular network of microtubules and actin microfilaments. Therefore, it is an excellent target for the development of new anti-parasitic drugs. Benzimidazole 2-carbamates, a class of well-known broad-spectrum anthelmintics, have been shown to inhibit the in vitro growth of many protozoa. Therefore, to find efficient anti-trypanosomal (trypanocidal) drugs, our group has designed and synthesised several benzimidazole derivatives. One, named JVG9 (5-chloro-1H-benzimidazole-2-thiol), has been found to be effective against T. cruzi bloodstream trypomastigotes under both in vitro and in vivo conditions. Here, we present the in vitro effects observed by laser scanning confocal and scanning electron microscopy on T. cruzi trypomastigotes. Changes in the surface and the distribution of the cytoskeletal proteins are consistent with the hypothesis that the trypanocidal activity of JVG9 involves the cytoskeleton as a target.
Original languageAmerican English
Pages (from-to)757-760
Number of pages4
JournalMemorias do Instituto Oswaldo Cruz
DOIs
StatePublished - 1 Sep 2014

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Trypanosoma cruzi
Cytoskeleton
Trypanocidal Agents
Anthelmintics
Cytoskeletal Proteins
Carbamates
Actin Cytoskeleton
Microtubules
Electron Scanning Microscopy
Lasers
Growth
Pharmaceutical Preparations
In Vitro Techniques
benzimidazole

Cite this

Díaz-Chiguer, Dylan L. ; Hernández-Luis, Francisco ; Nogueda-Torres, BenjamíN ; Castillo, Rafael ; Reynoso-Ducoing, Olivia ; Hernández-Campos, Alicia ; Ambrosio, Javier R. / JVG9, A benzimidazole derivative, Alters the surface and cytoskeleton of Trypanosoma cruzi bloodstream trypomastigotes. In: Memorias do Instituto Oswaldo Cruz. 2014 ; pp. 757-760.
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JVG9, A benzimidazole derivative, Alters the surface and cytoskeleton of Trypanosoma cruzi bloodstream trypomastigotes. / Díaz-Chiguer, Dylan L.; Hernández-Luis, Francisco; Nogueda-Torres, BenjamíN; Castillo, Rafael; Reynoso-Ducoing, Olivia; Hernández-Campos, Alicia; Ambrosio, Javier R.

In: Memorias do Instituto Oswaldo Cruz, 01.09.2014, p. 757-760.

Research output: Contribution to journalArticle

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AU - Díaz-Chiguer, Dylan L.

AU - Hernández-Luis, Francisco

AU - Nogueda-Torres, BenjamíN

AU - Castillo, Rafael

AU - Reynoso-Ducoing, Olivia

AU - Hernández-Campos, Alicia

AU - Ambrosio, Javier R.

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N2 - © 2014 Fundacao Oswaldo Cruz. All rights reserved. Trypanosoma cruzi has a particular cytoskeleton that consists of a subpellicular network of microtubules and actin microfilaments. Therefore, it is an excellent target for the development of new anti-parasitic drugs. Benzimidazole 2-carbamates, a class of well-known broad-spectrum anthelmintics, have been shown to inhibit the in vitro growth of many protozoa. Therefore, to find efficient anti-trypanosomal (trypanocidal) drugs, our group has designed and synthesised several benzimidazole derivatives. One, named JVG9 (5-chloro-1H-benzimidazole-2-thiol), has been found to be effective against T. cruzi bloodstream trypomastigotes under both in vitro and in vivo conditions. Here, we present the in vitro effects observed by laser scanning confocal and scanning electron microscopy on T. cruzi trypomastigotes. Changes in the surface and the distribution of the cytoskeletal proteins are consistent with the hypothesis that the trypanocidal activity of JVG9 involves the cytoskeleton as a target.

AB - © 2014 Fundacao Oswaldo Cruz. All rights reserved. Trypanosoma cruzi has a particular cytoskeleton that consists of a subpellicular network of microtubules and actin microfilaments. Therefore, it is an excellent target for the development of new anti-parasitic drugs. Benzimidazole 2-carbamates, a class of well-known broad-spectrum anthelmintics, have been shown to inhibit the in vitro growth of many protozoa. Therefore, to find efficient anti-trypanosomal (trypanocidal) drugs, our group has designed and synthesised several benzimidazole derivatives. One, named JVG9 (5-chloro-1H-benzimidazole-2-thiol), has been found to be effective against T. cruzi bloodstream trypomastigotes under both in vitro and in vivo conditions. Here, we present the in vitro effects observed by laser scanning confocal and scanning electron microscopy on T. cruzi trypomastigotes. Changes in the surface and the distribution of the cytoskeletal proteins are consistent with the hypothesis that the trypanocidal activity of JVG9 involves the cytoskeleton as a target.

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