TY - JOUR
T1 - JVG9, A benzimidazole derivative, Alters the surface and cytoskeleton of Trypanosoma cruzi bloodstream trypomastigotes
AU - Díaz-Chiguer, Dylan L.
AU - Hernández-Luis, Francisco
AU - Nogueda-Torres, BenjamíN
AU - Castillo, Rafael
AU - Reynoso-Ducoing, Olivia
AU - Hernández-Campos, Alicia
AU - Ambrosio, Javier R.
N1 - Publisher Copyright:
© 2014 Fundacao Oswaldo Cruz. All rights reserved.
PY - 2014/9/1
Y1 - 2014/9/1
N2 - Trypanosoma cruzi has a particular cytoskeleton that consists of a subpellicular network of microtubules and actin microfilaments. Therefore, it is an excellent target for the development of new anti-parasitic drugs. Benzimidazole 2-carbamates, a class of well-known broad-spectrum anthelmintics, have been shown to inhibit the in vitro growth of many protozoa. Therefore, to find efficient anti-trypanosomal (trypanocidal) drugs, our group has designed and synthesised several benzimidazole derivatives. One, named JVG9 (5-chloro-1H-benzimidazole-2-thiol), has been found to be effective against T. cruzi bloodstream trypomastigotes under both in vitro and in vivo conditions. Here, we present the in vitro effects observed by laser scanning confocal and scanning electron microscopy on T. cruzi trypomastigotes. Changes in the surface and the distribution of the cytoskeletal proteins are consistent with the hypothesis that the trypanocidal activity of JVG9 involves the cytoskeleton as a target.
AB - Trypanosoma cruzi has a particular cytoskeleton that consists of a subpellicular network of microtubules and actin microfilaments. Therefore, it is an excellent target for the development of new anti-parasitic drugs. Benzimidazole 2-carbamates, a class of well-known broad-spectrum anthelmintics, have been shown to inhibit the in vitro growth of many protozoa. Therefore, to find efficient anti-trypanosomal (trypanocidal) drugs, our group has designed and synthesised several benzimidazole derivatives. One, named JVG9 (5-chloro-1H-benzimidazole-2-thiol), has been found to be effective against T. cruzi bloodstream trypomastigotes under both in vitro and in vivo conditions. Here, we present the in vitro effects observed by laser scanning confocal and scanning electron microscopy on T. cruzi trypomastigotes. Changes in the surface and the distribution of the cytoskeletal proteins are consistent with the hypothesis that the trypanocidal activity of JVG9 involves the cytoskeleton as a target.
KW - Actin
KW - Benzimidazole
KW - Cytoskeleton
KW - Trypanosoma cruzi
KW - Tubulin
UR - http://www.scopus.com/inward/record.url?scp=84907227369&partnerID=8YFLogxK
U2 - 10.1590/0074-0276140096
DO - 10.1590/0074-0276140096
M3 - Artículo
SN - 0074-0276
VL - 109
SP - 757
EP - 760
JO - Memorias do Instituto Oswaldo Cruz
JF - Memorias do Instituto Oswaldo Cruz
IS - 6
ER -