TY - JOUR
T1 - Inhibitory effect of grapefruit juice on the genotoxicity induced by hydrogen peroxide in human lymphocytes
AU - Razo-Aguilera, G.
AU - Baez-Reyes, R.
AU - Álvarez-González, I.
AU - Paniagua-Pérez, R.
AU - Madrigal-Bujaidar, E.
PY - 2011/11
Y1 - 2011/11
N2 - By means of the comet assay we demonstrated a strong effect by hydrogen peroxide (HP) and no damage by grapefruit juice (GJ) in human lymphocytes. Cells exposed to HP and treated with three concentrations of GJ (10-90. min) showed an increase of DNA damage by HP over the control level, and a decrease of such damage by GJ. With the comet assay plus formamidopyrimidine-DNA-glycosylase we found the strongest increase of DNA damage by HP over the control level, and the strongest reduction of such damage by GJ. By applying the comet/FISH method we determined 98% of the p53 gene signals in the comet head of control cells along the experiment (10-90. min), in contrast with about 90% signals in the comet tail of cells exposed to HP. Cells treated with both agents showed a significant, concentration/time dependent return of p53 signals to the head, suggesting enhancement of the gene repair. Finally, with the annexin V assay we found an increase in apoptosis and necrosis by HP, and no effect by GJ; when GJ was added to HP treated cells no modification was observed in regard to apoptosis, although a decrease of necrosis was observed.
AB - By means of the comet assay we demonstrated a strong effect by hydrogen peroxide (HP) and no damage by grapefruit juice (GJ) in human lymphocytes. Cells exposed to HP and treated with three concentrations of GJ (10-90. min) showed an increase of DNA damage by HP over the control level, and a decrease of such damage by GJ. With the comet assay plus formamidopyrimidine-DNA-glycosylase we found the strongest increase of DNA damage by HP over the control level, and the strongest reduction of such damage by GJ. By applying the comet/FISH method we determined 98% of the p53 gene signals in the comet head of control cells along the experiment (10-90. min), in contrast with about 90% signals in the comet tail of cells exposed to HP. Cells treated with both agents showed a significant, concentration/time dependent return of p53 signals to the head, suggesting enhancement of the gene repair. Finally, with the annexin V assay we found an increase in apoptosis and necrosis by HP, and no effect by GJ; when GJ was added to HP treated cells no modification was observed in regard to apoptosis, although a decrease of necrosis was observed.
KW - Antigenotoxicity mechanism
KW - Antioxidant
KW - Comet-FISH
KW - Grapefruit juice
UR - http://www.scopus.com/inward/record.url?scp=80053571403&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2011.07.003
DO - 10.1016/j.fct.2011.07.003
M3 - Artículo
C2 - 21777646
SN - 0278-6915
VL - 49
SP - 2947
EP - 2953
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 11
ER -