Enhanced sulfate reduction and trichloroethylene (TCE) biodegradation in a UASB reactor operated with a sludge developed from hydrothermal vents sediments: Process and microbial ecology

Claudia Guerrero-Barajas, Alberto Ordaz, Claudio Garibay-Orijel, Selene Montserrat García-Solares, Fernando Bastida-González, Paola Berenice Zárate-Segura

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Abstract

Most Trichloroethylene (TCE) biodegradation reports refer to methanogenic conditions, however, in this work, enhanced sulfidogenesis and TCE biodegradation were achieved in an upflow anaerobic sludge blanket (UASB) reactor in which a completely sulfidogenic sludge, from hydrothermal vents sediments, was developed. The work was divided in three stages, (i) sludge development and sulfate reducing activity (SRA) evaluation, (ii) TCE biodegradation and (iii) SRA evaluation after TCE biodegradation. For (i) SR was 98±0.1%, 84% as sulfide (H2S, 1200±28mg/L), sulfate reducing activity (SRA) was 188±50mg COD H2S/g VSS*d. For (ii) The reactor reached 74% of TCE removal, concentrations of vinyl chloride of 16±0.3μM (5% of the TCE added) and ethene 202±81μM (67% of the TCE added), SRA of 161±7mg COD H2S/g VSS*d, 68% of sulfide (H2S) production and 93% of COD removal. For (iii) SRA was of 248±22mg COD H2S/g VSS*d demonstrating no adverse effects due to TCE.Among the genera of the microorganisms identified in the sludge during TCE biodegradation were: Dehalobacter, Desulfotomaculum, Sulfospirillum, Desulfitobacterium, Desulfovibrio and Clostridium. To the best of our knowledge, this is the first report using a sulfidogenic UASB reactor to biodegrade TCE. The overall conclusions of this work are that the reactor is efficient on both, sulfate and TCE biodegradation and it could be used to decontaminate wastewater containing organic solvents and relatively high concentrations of sulfate. © 2014 Elsevier Ltd.
Original languageAmerican English
Pages (from-to)182-191
Number of pages162
JournalInternational Biodeterioration and Biodegradation
DOIs
StatePublished - 1 Jan 2014

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Hydrothermal Vents
Trichloroethylene
microbial ecology
Vents
hydrothermal vent
Ecology
trichloroethylene
Sewage
Biodegradation
Sulfates
biodegradation
Sediments
sludge
sulfate
sediment
Sulfides
Desulfitobacterium
Desulfotomaculum
reactor
Desulfovibrio

Cite this

@article{a6a57547e3374d9083b728533148c82b,
title = "Enhanced sulfate reduction and trichloroethylene (TCE) biodegradation in a UASB reactor operated with a sludge developed from hydrothermal vents sediments: Process and microbial ecology",
abstract = "Most Trichloroethylene (TCE) biodegradation reports refer to methanogenic conditions, however, in this work, enhanced sulfidogenesis and TCE biodegradation were achieved in an upflow anaerobic sludge blanket (UASB) reactor in which a completely sulfidogenic sludge, from hydrothermal vents sediments, was developed. The work was divided in three stages, (i) sludge development and sulfate reducing activity (SRA) evaluation, (ii) TCE biodegradation and (iii) SRA evaluation after TCE biodegradation. For (i) SR was 98±0.1{\%}, 84{\%} as sulfide (H2S, 1200±28mg/L), sulfate reducing activity (SRA) was 188±50mg COD H2S/g VSS*d. For (ii) The reactor reached 74{\%} of TCE removal, concentrations of vinyl chloride of 16±0.3μM (5{\%} of the TCE added) and ethene 202±81μM (67{\%} of the TCE added), SRA of 161±7mg COD H2S/g VSS*d, 68{\%} of sulfide (H2S) production and 93{\%} of COD removal. For (iii) SRA was of 248±22mg COD H2S/g VSS*d demonstrating no adverse effects due to TCE.Among the genera of the microorganisms identified in the sludge during TCE biodegradation were: Dehalobacter, Desulfotomaculum, Sulfospirillum, Desulfitobacterium, Desulfovibrio and Clostridium. To the best of our knowledge, this is the first report using a sulfidogenic UASB reactor to biodegrade TCE. The overall conclusions of this work are that the reactor is efficient on both, sulfate and TCE biodegradation and it could be used to decontaminate wastewater containing organic solvents and relatively high concentrations of sulfate. {\circledC} 2014 Elsevier Ltd.",
author = "Claudia Guerrero-Barajas and Alberto Ordaz and Claudio Garibay-Orijel and Garc{\'i}a-Solares, {Selene Montserrat} and Fernando Bastida-Gonz{\'a}lez and Z{\'a}rate-Segura, {Paola Berenice}",
year = "2014",
month = "1",
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doi = "10.1016/j.ibiod.2014.07.015",
language = "American English",
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TY - JOUR

T1 - Enhanced sulfate reduction and trichloroethylene (TCE) biodegradation in a UASB reactor operated with a sludge developed from hydrothermal vents sediments: Process and microbial ecology

AU - Guerrero-Barajas, Claudia

AU - Ordaz, Alberto

AU - Garibay-Orijel, Claudio

AU - García-Solares, Selene Montserrat

AU - Bastida-González, Fernando

AU - Zárate-Segura, Paola Berenice

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Most Trichloroethylene (TCE) biodegradation reports refer to methanogenic conditions, however, in this work, enhanced sulfidogenesis and TCE biodegradation were achieved in an upflow anaerobic sludge blanket (UASB) reactor in which a completely sulfidogenic sludge, from hydrothermal vents sediments, was developed. The work was divided in three stages, (i) sludge development and sulfate reducing activity (SRA) evaluation, (ii) TCE biodegradation and (iii) SRA evaluation after TCE biodegradation. For (i) SR was 98±0.1%, 84% as sulfide (H2S, 1200±28mg/L), sulfate reducing activity (SRA) was 188±50mg COD H2S/g VSS*d. For (ii) The reactor reached 74% of TCE removal, concentrations of vinyl chloride of 16±0.3μM (5% of the TCE added) and ethene 202±81μM (67% of the TCE added), SRA of 161±7mg COD H2S/g VSS*d, 68% of sulfide (H2S) production and 93% of COD removal. For (iii) SRA was of 248±22mg COD H2S/g VSS*d demonstrating no adverse effects due to TCE.Among the genera of the microorganisms identified in the sludge during TCE biodegradation were: Dehalobacter, Desulfotomaculum, Sulfospirillum, Desulfitobacterium, Desulfovibrio and Clostridium. To the best of our knowledge, this is the first report using a sulfidogenic UASB reactor to biodegrade TCE. The overall conclusions of this work are that the reactor is efficient on both, sulfate and TCE biodegradation and it could be used to decontaminate wastewater containing organic solvents and relatively high concentrations of sulfate. © 2014 Elsevier Ltd.

AB - Most Trichloroethylene (TCE) biodegradation reports refer to methanogenic conditions, however, in this work, enhanced sulfidogenesis and TCE biodegradation were achieved in an upflow anaerobic sludge blanket (UASB) reactor in which a completely sulfidogenic sludge, from hydrothermal vents sediments, was developed. The work was divided in three stages, (i) sludge development and sulfate reducing activity (SRA) evaluation, (ii) TCE biodegradation and (iii) SRA evaluation after TCE biodegradation. For (i) SR was 98±0.1%, 84% as sulfide (H2S, 1200±28mg/L), sulfate reducing activity (SRA) was 188±50mg COD H2S/g VSS*d. For (ii) The reactor reached 74% of TCE removal, concentrations of vinyl chloride of 16±0.3μM (5% of the TCE added) and ethene 202±81μM (67% of the TCE added), SRA of 161±7mg COD H2S/g VSS*d, 68% of sulfide (H2S) production and 93% of COD removal. For (iii) SRA was of 248±22mg COD H2S/g VSS*d demonstrating no adverse effects due to TCE.Among the genera of the microorganisms identified in the sludge during TCE biodegradation were: Dehalobacter, Desulfotomaculum, Sulfospirillum, Desulfitobacterium, Desulfovibrio and Clostridium. To the best of our knowledge, this is the first report using a sulfidogenic UASB reactor to biodegrade TCE. The overall conclusions of this work are that the reactor is efficient on both, sulfate and TCE biodegradation and it could be used to decontaminate wastewater containing organic solvents and relatively high concentrations of sulfate. © 2014 Elsevier Ltd.

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