TY - JOUR
T1 - Enhanced sulfate reduction and trichloroethylene (TCE) biodegradation in a UASB reactor operated with a sludge developed from hydrothermal vents sediments
T2 - Process and microbial ecology
AU - Guerrero-Barajas, Claudia
AU - Ordaz, Alberto
AU - Garibay-Orijel, Claudio
AU - García-Solares, Selene Montserrat
AU - Bastida-González, Fernando
AU - Zárate-Segura, Paola Berenice
N1 - Funding Information:
The authors are grateful for the financial support provided by Instituto Politécnico Nacional grants 20120110 , 20130399 and 20140239 SIP and also by Instituto de Ciencia y Tecnología del Distrito Federal Mexico (grant awarded to Guerrero-Barajas Claudia, PICS 08-79 , ICYT-DF, 2009-2012 . This grant supported the collection of the sediments and the experimental work from 2009 to 2011). Thanks also to CONACYT – Mexico for the graduate scholarship (225806) awarded to Selene Montserrat García-Solares and for the financial support provided by grant 82627 . Also we thank the technical assistance of Javier Pareja Camacho.
PY - 2014/10
Y1 - 2014/10
N2 - Most Trichloroethylene (TCE) biodegradation reports refer to methanogenic conditions, however, in this work, enhanced sulfidogenesis and TCE biodegradation were achieved in an upflow anaerobic sludge blanket (UASB) reactor in which a completely sulfidogenic sludge, from hydrothermal vents sediments, was developed. The work was divided in three stages, (i) sludge development and sulfate reducing activity (SRA) evaluation, (ii) TCE biodegradation and (iii) SRA evaluation after TCE biodegradation. For (i) SR was 98±0.1%, 84% as sulfide (H2S, 1200±28mg/L), sulfate reducing activity (SRA) was 188±50mg COD H2S/g VSS*d. For (ii) The reactor reached 74% of TCE removal, concentrations of vinyl chloride of 16±0.3μM (5% of the TCE added) and ethene 202±81μM (67% of the TCE added), SRA of 161±7mg COD H2S/g VSS*d, 68% of sulfide (H2S) production and 93% of COD removal. For (iii) SRA was of 248±22mg COD H2S/g VSS*d demonstrating no adverse effects due to TCE.Among the genera of the microorganisms identified in the sludge during TCE biodegradation were: Dehalobacter, Desulfotomaculum, Sulfospirillum, Desulfitobacterium, Desulfovibrio and Clostridium. To the best of our knowledge, this is the first report using a sulfidogenic UASB reactor to biodegrade TCE. The overall conclusions of this work are that the reactor is efficient on both, sulfate and TCE biodegradation and it could be used to decontaminate wastewater containing organic solvents and relatively high concentrations of sulfate.
AB - Most Trichloroethylene (TCE) biodegradation reports refer to methanogenic conditions, however, in this work, enhanced sulfidogenesis and TCE biodegradation were achieved in an upflow anaerobic sludge blanket (UASB) reactor in which a completely sulfidogenic sludge, from hydrothermal vents sediments, was developed. The work was divided in three stages, (i) sludge development and sulfate reducing activity (SRA) evaluation, (ii) TCE biodegradation and (iii) SRA evaluation after TCE biodegradation. For (i) SR was 98±0.1%, 84% as sulfide (H2S, 1200±28mg/L), sulfate reducing activity (SRA) was 188±50mg COD H2S/g VSS*d. For (ii) The reactor reached 74% of TCE removal, concentrations of vinyl chloride of 16±0.3μM (5% of the TCE added) and ethene 202±81μM (67% of the TCE added), SRA of 161±7mg COD H2S/g VSS*d, 68% of sulfide (H2S) production and 93% of COD removal. For (iii) SRA was of 248±22mg COD H2S/g VSS*d demonstrating no adverse effects due to TCE.Among the genera of the microorganisms identified in the sludge during TCE biodegradation were: Dehalobacter, Desulfotomaculum, Sulfospirillum, Desulfitobacterium, Desulfovibrio and Clostridium. To the best of our knowledge, this is the first report using a sulfidogenic UASB reactor to biodegrade TCE. The overall conclusions of this work are that the reactor is efficient on both, sulfate and TCE biodegradation and it could be used to decontaminate wastewater containing organic solvents and relatively high concentrations of sulfate.
KW - Clostridium sp
KW - Dehalorespiring bacteria
KW - Desulfovibrio sp.
KW - Sulfate reducing bacteria
KW - Sulfidogenic UASB reactors
KW - Trichloroethylene biodegradation
UR - http://www.scopus.com/inward/record.url?scp=84907058401&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2014.07.015
DO - 10.1016/j.ibiod.2014.07.015
M3 - Artículo
SN - 0964-8305
VL - 94
SP - 182
EP - 191
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
ER -