TY - JOUR
T1 - Engineering concanavalin b to release bioactive peptides against metabolic syndrome
AU - Maldonado-Torres, Diego Armando
AU - Jara-Romero, G. Janet
AU - Rosas-Cárdenas, Flor de Fátima
AU - Fernández-Velasco, D. Alejandro
AU - Luna-Suárez, Silvia
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/7
Y1 - 2021/7
N2 - Metabolic syndrome is a severe public health issue characterized by multiple metabolic disturbances. Current treatments prescribe a particular drug for each of them, producing multiple side effects. As a first step towards a more integral approach, we applied our recently described methodology to design single proteins, based in the Concanavalin B scaffold (1CNV), that contain several bioactive peptides (BPs), including antioxidant and lipid-lowering activities as well as inhibitors of dipeptidyl peptidase IV (DPPIV) and the angiotensin converting enzyme. Modified Concanavalin (CNV44), the designed protein that showed the best in silico properties, was expressed in high yields in E. coli and purified to homogeneity. After in vitro digestion with gastrointestinal enzymes, all the biological activities tested where higher in CNV44 when compared to the non-modified protein 1CNV, or to other previous reports. The results presented here represent the first in vitro evidence of a modified protein with the potential to treat metabolic syndrome and open the venue for the design of proteins to treat other non-communicable diseases.
AB - Metabolic syndrome is a severe public health issue characterized by multiple metabolic disturbances. Current treatments prescribe a particular drug for each of them, producing multiple side effects. As a first step towards a more integral approach, we applied our recently described methodology to design single proteins, based in the Concanavalin B scaffold (1CNV), that contain several bioactive peptides (BPs), including antioxidant and lipid-lowering activities as well as inhibitors of dipeptidyl peptidase IV (DPPIV) and the angiotensin converting enzyme. Modified Concanavalin (CNV44), the designed protein that showed the best in silico properties, was expressed in high yields in E. coli and purified to homogeneity. After in vitro digestion with gastrointestinal enzymes, all the biological activities tested where higher in CNV44 when compared to the non-modified protein 1CNV, or to other previous reports. The results presented here represent the first in vitro evidence of a modified protein with the potential to treat metabolic syndrome and open the venue for the design of proteins to treat other non-communicable diseases.
KW - ACEI
KW - Antioxidant activity
KW - Bioactive peptides
KW - DPPIV
KW - Lipid-lowering
KW - Metabolic syndrome
KW - Protein engineering
UR - http://www.scopus.com/inward/record.url?scp=85110768633&partnerID=8YFLogxK
U2 - 10.3390/foods10071554
DO - 10.3390/foods10071554
M3 - Artículo
C2 - 34359424
AN - SCOPUS:85110768633
SN - 2304-8158
VL - 10
JO - Foods
JF - Foods
IS - 7
M1 - 1554
ER -