Deterministic and probabilistic predictive microbiology-based indicator of the listeriosis and microbial spoilage risk of pasteurized milk stored in residential refrigerators

Veronica Rodriguez-Martinez, Gonzalo Velázquez, Rosario de Jesús Rodríguez Altaif, Fabian Fagotti, Jorge Welti-Chanes, J. Antonio Torres

Research output: Contribution to journalArticle

Abstract

Following deterministic and probabilistic predictive microbiology procedures, cumulative 48 h exponential growth estimates were obtained for Listeria monocytogenes (Lm) and Pseudomonas putida (Pp) as preservation effectiveness indicators for pasteurized milk stored in the middle door shelf of a residential refrigerator set at 5 °C. Experimental factors studied were refrigerator load, door openings, compressor operating in variable (VS) or ON/OFF single speed (SS) mode, room temperature (TChamber = 32.2/21.1 °C), and milk exposure to TChamber (replicating product use). The 32.2 °C/high load condition yielded deterministic 48 h exponential growth estimates (ΔlogN48h, log10 N CFU/mL) of 1.6 ± 0.1/1.5 ± 0.1 for Lm and 5.0 ± 0.3/4.7 ± 0.3 for Pp (replicate-1/replicate-2, VS, milk kept in refrigerator). The corresponding 5–95% probabilistic ΔlogN48h intervals were 1.0–2.3 and 3.4–6.6, respectively. Considering new technologies seeking to lower the temperature of products stored in door shelves, the analysis was repeated using simulated data obtained by lowering experimental temperatures by Tred (°C), a value determined for each test condition and keeping milk above 2 °C with 90% certainty. For the same previous conditions, deterministic ΔlogN48h estimates for temperatures lowered by Tred= 4.5–4.8 °C, yielded ΔlogN48h of 0.06 ± 0.0 for Lm and 2.6 ± 0.1 for Pp. These results highlight the importance of improving the temperature control of residential refrigerator door shelves.

Original languageEnglish
Article number108650
JournalLWT
Volume117
DOIs
StatePublished - Jan 2020
Externally publishedYes

Fingerprint

predictive microbiology
Listeriosis
listeriosis
pasteurized milk
refrigerators
Microbiology
spoilage
Pseudomonas putida
Milk
Listeria monocytogenes
Temperature
milk
temperature
compressors
stored products
ambient temperature
Growth
Technology
testing

Keywords

  • Listeria monocytogenes
  • Pasteurized milk
  • Predictive microbiology
  • Pseudomonas putida
  • Residential refrigerator temperature

Cite this

Rodriguez-Martinez, Veronica ; Velázquez, Gonzalo ; de Jesús Rodríguez Altaif, Rosario ; Fagotti, Fabian ; Welti-Chanes, Jorge ; Torres, J. Antonio. / Deterministic and probabilistic predictive microbiology-based indicator of the listeriosis and microbial spoilage risk of pasteurized milk stored in residential refrigerators. In: LWT. 2020 ; Vol. 117.
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abstract = "Following deterministic and probabilistic predictive microbiology procedures, cumulative 48 h exponential growth estimates were obtained for Listeria monocytogenes (Lm) and Pseudomonas putida (Pp) as preservation effectiveness indicators for pasteurized milk stored in the middle door shelf of a residential refrigerator set at 5 °C. Experimental factors studied were refrigerator load, door openings, compressor operating in variable (VS) or ON/OFF single speed (SS) mode, room temperature (TChamber = 32.2/21.1 °C), and milk exposure to TChamber (replicating product use). The 32.2 °C/high load condition yielded deterministic 48 h exponential growth estimates (ΔlogN48h, log10 N CFU/mL) of 1.6 ± 0.1/1.5 ± 0.1 for Lm and 5.0 ± 0.3/4.7 ± 0.3 for Pp (replicate-1/replicate-2, VS, milk kept in refrigerator). The corresponding 5–95{\%} probabilistic ΔlogN48h intervals were 1.0–2.3 and 3.4–6.6, respectively. Considering new technologies seeking to lower the temperature of products stored in door shelves, the analysis was repeated using simulated data obtained by lowering experimental temperatures by Tred (°C), a value determined for each test condition and keeping milk above 2 °C with 90{\%} certainty. For the same previous conditions, deterministic ΔlogN48h estimates for temperatures lowered by Tred= 4.5–4.8 °C, yielded ΔlogN48h of 0.06 ± 0.0 for Lm and 2.6 ± 0.1 for Pp. These results highlight the importance of improving the temperature control of residential refrigerator door shelves.",
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Deterministic and probabilistic predictive microbiology-based indicator of the listeriosis and microbial spoilage risk of pasteurized milk stored in residential refrigerators. / Rodriguez-Martinez, Veronica; Velázquez, Gonzalo; de Jesús Rodríguez Altaif, Rosario; Fagotti, Fabian; Welti-Chanes, Jorge; Torres, J. Antonio.

In: LWT, Vol. 117, 108650, 01.2020.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Deterministic and probabilistic predictive microbiology-based indicator of the listeriosis and microbial spoilage risk of pasteurized milk stored in residential refrigerators

AU - Rodriguez-Martinez, Veronica

AU - Velázquez, Gonzalo

AU - de Jesús Rodríguez Altaif, Rosario

AU - Fagotti, Fabian

AU - Welti-Chanes, Jorge

AU - Torres, J. Antonio

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N2 - Following deterministic and probabilistic predictive microbiology procedures, cumulative 48 h exponential growth estimates were obtained for Listeria monocytogenes (Lm) and Pseudomonas putida (Pp) as preservation effectiveness indicators for pasteurized milk stored in the middle door shelf of a residential refrigerator set at 5 °C. Experimental factors studied were refrigerator load, door openings, compressor operating in variable (VS) or ON/OFF single speed (SS) mode, room temperature (TChamber = 32.2/21.1 °C), and milk exposure to TChamber (replicating product use). The 32.2 °C/high load condition yielded deterministic 48 h exponential growth estimates (ΔlogN48h, log10 N CFU/mL) of 1.6 ± 0.1/1.5 ± 0.1 for Lm and 5.0 ± 0.3/4.7 ± 0.3 for Pp (replicate-1/replicate-2, VS, milk kept in refrigerator). The corresponding 5–95% probabilistic ΔlogN48h intervals were 1.0–2.3 and 3.4–6.6, respectively. Considering new technologies seeking to lower the temperature of products stored in door shelves, the analysis was repeated using simulated data obtained by lowering experimental temperatures by Tred (°C), a value determined for each test condition and keeping milk above 2 °C with 90% certainty. For the same previous conditions, deterministic ΔlogN48h estimates for temperatures lowered by Tred= 4.5–4.8 °C, yielded ΔlogN48h of 0.06 ± 0.0 for Lm and 2.6 ± 0.1 for Pp. These results highlight the importance of improving the temperature control of residential refrigerator door shelves.

AB - Following deterministic and probabilistic predictive microbiology procedures, cumulative 48 h exponential growth estimates were obtained for Listeria monocytogenes (Lm) and Pseudomonas putida (Pp) as preservation effectiveness indicators for pasteurized milk stored in the middle door shelf of a residential refrigerator set at 5 °C. Experimental factors studied were refrigerator load, door openings, compressor operating in variable (VS) or ON/OFF single speed (SS) mode, room temperature (TChamber = 32.2/21.1 °C), and milk exposure to TChamber (replicating product use). The 32.2 °C/high load condition yielded deterministic 48 h exponential growth estimates (ΔlogN48h, log10 N CFU/mL) of 1.6 ± 0.1/1.5 ± 0.1 for Lm and 5.0 ± 0.3/4.7 ± 0.3 for Pp (replicate-1/replicate-2, VS, milk kept in refrigerator). The corresponding 5–95% probabilistic ΔlogN48h intervals were 1.0–2.3 and 3.4–6.6, respectively. Considering new technologies seeking to lower the temperature of products stored in door shelves, the analysis was repeated using simulated data obtained by lowering experimental temperatures by Tred (°C), a value determined for each test condition and keeping milk above 2 °C with 90% certainty. For the same previous conditions, deterministic ΔlogN48h estimates for temperatures lowered by Tred= 4.5–4.8 °C, yielded ΔlogN48h of 0.06 ± 0.0 for Lm and 2.6 ± 0.1 for Pp. These results highlight the importance of improving the temperature control of residential refrigerator door shelves.

KW - Listeria monocytogenes

KW - Pasteurized milk

KW - Predictive microbiology

KW - Pseudomonas putida

KW - Residential refrigerator temperature

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