TY - JOUR
T1 - Deterministic and probabilistic predictive microbiology-based indicator of the listeriosis and microbial spoilage risk of pasteurized milk stored in residential refrigerators
AU - Rodriguez-Martinez, Veronica
AU - Velázquez, Gonzalo
AU - de Jesús Rodríguez Altaif, Rosario
AU - Fagotti, Fabian
AU - Welti-Chanes, Jorge
AU - Torres, J. Antonio
N1 - Funding Information:
Authors acknowledge the support from Tecnológico de Monterrey, Mexico (Research Chair Funds GEE 1A01001 and CDB081 ) and Nidec Global Appliance México, S. de R.L. de C.V. , as well as the experimental contributions from Alejandro L. Escobar-Zamarron.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Following deterministic and probabilistic predictive microbiology procedures, cumulative 48 h exponential growth estimates were obtained for Listeria monocytogenes (Lm) and Pseudomonas putida (Pp) as preservation effectiveness indicators for pasteurized milk stored in the middle door shelf of a residential refrigerator set at 5 °C. Experimental factors studied were refrigerator load, door openings, compressor operating in variable (VS) or ON/OFF single speed (SS) mode, room temperature (TChamber = 32.2/21.1 °C), and milk exposure to TChamber (replicating product use). The 32.2 °C/high load condition yielded deterministic 48 h exponential growth estimates (ΔlogN48h, log10 N CFU/mL) of 1.6 ± 0.1/1.5 ± 0.1 for Lm and 5.0 ± 0.3/4.7 ± 0.3 for Pp (replicate-1/replicate-2, VS, milk kept in refrigerator). The corresponding 5–95% probabilistic ΔlogN48h intervals were 1.0–2.3 and 3.4–6.6, respectively. Considering new technologies seeking to lower the temperature of products stored in door shelves, the analysis was repeated using simulated data obtained by lowering experimental temperatures by Tred (°C), a value determined for each test condition and keeping milk above 2 °C with 90% certainty. For the same previous conditions, deterministic ΔlogN48h estimates for temperatures lowered by Tred= 4.5–4.8 °C, yielded ΔlogN48h of 0.06 ± 0.0 for Lm and 2.6 ± 0.1 for Pp. These results highlight the importance of improving the temperature control of residential refrigerator door shelves.
AB - Following deterministic and probabilistic predictive microbiology procedures, cumulative 48 h exponential growth estimates were obtained for Listeria monocytogenes (Lm) and Pseudomonas putida (Pp) as preservation effectiveness indicators for pasteurized milk stored in the middle door shelf of a residential refrigerator set at 5 °C. Experimental factors studied were refrigerator load, door openings, compressor operating in variable (VS) or ON/OFF single speed (SS) mode, room temperature (TChamber = 32.2/21.1 °C), and milk exposure to TChamber (replicating product use). The 32.2 °C/high load condition yielded deterministic 48 h exponential growth estimates (ΔlogN48h, log10 N CFU/mL) of 1.6 ± 0.1/1.5 ± 0.1 for Lm and 5.0 ± 0.3/4.7 ± 0.3 for Pp (replicate-1/replicate-2, VS, milk kept in refrigerator). The corresponding 5–95% probabilistic ΔlogN48h intervals were 1.0–2.3 and 3.4–6.6, respectively. Considering new technologies seeking to lower the temperature of products stored in door shelves, the analysis was repeated using simulated data obtained by lowering experimental temperatures by Tred (°C), a value determined for each test condition and keeping milk above 2 °C with 90% certainty. For the same previous conditions, deterministic ΔlogN48h estimates for temperatures lowered by Tred= 4.5–4.8 °C, yielded ΔlogN48h of 0.06 ± 0.0 for Lm and 2.6 ± 0.1 for Pp. These results highlight the importance of improving the temperature control of residential refrigerator door shelves.
KW - Listeria monocytogenes
KW - Pasteurized milk
KW - Predictive microbiology
KW - Pseudomonas putida
KW - Residential refrigerator temperature
UR - http://www.scopus.com/inward/record.url?scp=85072773987&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/e3157a0a-e77a-3834-bf33-b75a4f761f6a/
U2 - 10.1016/j.lwt.2019.108650
DO - 10.1016/j.lwt.2019.108650
M3 - Artículo
AN - SCOPUS:85072773987
SN - 0023-6438
VL - 117
JO - LWT
JF - LWT
M1 - 108650
ER -