Determination of Salmonella Typhimurium by Fourier Transform Infrared (FTIR) Spectroscopy Using a Hydrogenated Amorphous Silicon Carbide (a-SiC:H) Thin Film

Francisco Javier Gómez-Montaño, Abdú Orduña-Díaz, María del Carmen Guadalupe Avelino-Flores, Fabiola Avelino-Flores, Claudia Reyes-Betanzo, Marlon Rojas-López

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Salmonella is one of the main microorganisms that causes food-borne illnesses worldwide, although there are standard procedures to determine its presence or absence in food samples. However, these methodologies are time consuming and cumbersome, so the development of devices that rapidly and accurately determine bacteria is necessary. Various materials have been used to construct these devices. Hydrogenated amorphous silicon carbide (a-SiC:H) is one of the least studied. Therefore, in the present work, self-assembled monolayers were applied on a-SiC:H thin films to determine Salmonella Typhimurium ATCC 14028 by Fourier transform infrared spectroscopy. The detection of this microorganism was performed between 1060 and 960 cm−1 corresponding to carbohydrate and phosphate groups of the bacteria. The lowest detected concentration was 10 CFU/mL. This work confirms the utility of a-SiC:H to reliably and rapidly determine Salmonella.

Original languageEnglish
Pages (from-to)2655-2665
Number of pages11
JournalAnalytical Letters
Volume54
Issue number16
DOIs
StatePublished - 2021

Keywords

  • Fourier transform infrared (FTIR) spectroscopy
  • Salmonella
  • hydrogenated amorphous silicon carbide (a-SiC:H)
  • immunosensor
  • self-assembled monolayers (SAM)
  • thin films

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