Characterization of digestive gland esterase-lipase activity of juvenile redclaw crayfish Cherax quadricarinatus

Silverio López-López; Héctor Nolasco; Fernando Vega-Villasante

doi:10.1016/S1096-4959(03)00087-3

Characterization of digestive gland esterase-lipase activity of juvenile redclaw crayfish Cherax quadricarinatus

Silverio López-López, Héctor Nolasco, Fernando Vega-Villasante

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Investigation of esterase-lipase activity in the digestive gland of redclaw crayfish Cherax quadricarinatus showed that the optimum enzyme activity occurred between 35 and 40 °C, with 100 mM NaCl at pH 8.5. Heavy metals completely inhibited and calcium ions partially inhibited enzyme activity. The enzyme activity diminished as the length of the fatty acid chain of substrates increased. Molecular masses for four isozymes were 43, 46, 63 and 118 kDa, respectively, as determined by PAGE.

Original language	English
Pages (from-to)	337-347
Number of pages	11
Journal	Comparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume	135
Issue number	2
DOIs	https://doi.org/10.1016/S1096-4959(03)00087-3
State	Published - 1 Jun 2003
Externally published	Yes

Keywords

Cherax quadricarinatus
Crayfish
Digestive gland
Esterase
Lipase
Molecular mass
Parastacidae
Red claw

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10.1016/S1096-4959(03)00087-3

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title = "Characterization of digestive gland esterase-lipase activity of juvenile redclaw crayfish Cherax quadricarinatus",

abstract = "Investigation of esterase-lipase activity in the digestive gland of redclaw crayfish Cherax quadricarinatus showed that the optimum enzyme activity occurred between 35 and 40 °C, with 100 mM NaCl at pH 8.5. Heavy metals completely inhibited and calcium ions partially inhibited enzyme activity. The enzyme activity diminished as the length of the fatty acid chain of substrates increased. Molecular masses for four isozymes were 43, 46, 63 and 118 kDa, respectively, as determined by PAGE.",

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note = "Funding Information: This study is part of the doctoral research of SLL funded by the Consejo Nacional de Ciencia y Tecnolog{\'ı}a, Mexico (CONACyT Grant 2888-1). Thanks to Humberto Villarreal for co-direction of the research project and donating specimens. Ismael Valdivia and Francisco Encarnaci{\'o}n of the CIBNOR nutrition laboratory provided technical support, and editing staff at CIBNOR improved the text.",

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AU - López-López, Silverio

AU - Nolasco, Héctor

AU - Vega-Villasante, Fernando

N1 - Funding Information: This study is part of the doctoral research of SLL funded by the Consejo Nacional de Ciencia y Tecnologı́a, Mexico (CONACyT Grant 2888-1). Thanks to Humberto Villarreal for co-direction of the research project and donating specimens. Ismael Valdivia and Francisco Encarnación of the CIBNOR nutrition laboratory provided technical support, and editing staff at CIBNOR improved the text.

PY - 2003/6/1

Y1 - 2003/6/1

N2 - Investigation of esterase-lipase activity in the digestive gland of redclaw crayfish Cherax quadricarinatus showed that the optimum enzyme activity occurred between 35 and 40 °C, with 100 mM NaCl at pH 8.5. Heavy metals completely inhibited and calcium ions partially inhibited enzyme activity. The enzyme activity diminished as the length of the fatty acid chain of substrates increased. Molecular masses for four isozymes were 43, 46, 63 and 118 kDa, respectively, as determined by PAGE.

AB - Investigation of esterase-lipase activity in the digestive gland of redclaw crayfish Cherax quadricarinatus showed that the optimum enzyme activity occurred between 35 and 40 °C, with 100 mM NaCl at pH 8.5. Heavy metals completely inhibited and calcium ions partially inhibited enzyme activity. The enzyme activity diminished as the length of the fatty acid chain of substrates increased. Molecular masses for four isozymes were 43, 46, 63 and 118 kDa, respectively, as determined by PAGE.

KW - Cherax quadricarinatus

KW - Crayfish

KW - Digestive gland

KW - Esterase

KW - Lipase

KW - Molecular mass

KW - Parastacidae

KW - Red claw

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U2 - 10.1016/S1096-4959(03)00087-3

DO - 10.1016/S1096-4959(03)00087-3

M3 - Artículo

SN - 1096-4959

VL - 135

SP - 337

EP - 347

JO - Comparative Biochemistry and Physiology -- Part B: Biochemistry and

JF - Comparative Biochemistry and Physiology -- Part B: Biochemistry and

IS - 2

ER -

Characterization of digestive gland esterase-lipase activity of juvenile redclaw crayfish Cherax quadricarinatus

Abstract

Keywords

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