TY - JOUR
T1 - Antihypertensive activity of AMC3, an engineered 11S amaranth globulin expressed in Escherichia coli, in spontaneously hypertensive rats
AU - Medina-Godoy, Sergio
AU - Rodríguez-Yáñez, Silvia K.
AU - Bobadilla, Norma A.
AU - Pérez-Villalva, Rosalba
AU - Valdez-Ortiz, Rafael
AU - Hong, Enrique
AU - Luna-Suárez, Silvia
AU - Paredes-López, Octavio
AU - Valdez-Ortiz, Angel
N1 - Funding Information:
We thank the technical assistance of Joyce Trujillo during the performance of this study. This project was supported by grants from Consejo Nacional de Ciencia y Tecnología (CONACyT, 58791 and 101030 ) and Universidad Autónoma de Sinaloa ( PROFAPI 2007/069 ). We are grateful to Jean Pierre Le Caer and Olinda Gimello of ICSN-CNRS-Equipe de spectrométrie de masse for MSMS analysis.
PY - 2013/7
Y1 - 2013/7
N2 - The acidic-subunit of 11S seed storage globulin of Amaranthus hypochondriacus was recently modified by the incorporation of antihypertensive biopeptides: four units of Val-Tyr dipeptides (VY) in tandem and one of Ile-Pro-Pro tripeptide (IPP), was named AMC3. The in vivo effect of AMC3 enzymatic hydrolysates (AEH) produced in Escherichia coli, was evaluated in spontaneously hypertensive rats (SHR) by once-oral administration experiments. As positive control a SHR group received a dose of captopril (50. mg/kg) (an angiotensin-converting enzyme (ACE) inhibitor used for the treatment of hypertension). Mean arterial pressure (MAP) was measured for 6. h after AEH or captopril administration. AEH administration at maximal dose (100. mg/kg) significantly reduced MAP similar to the group treated with captopril. The maximal reduction in MAP was observed after 3.5. h after AEH oral administration. The present study showed that enzymatic hydrolysates of AMC3 containing ACE inhibitory peptides (4xVY and IPP) sequences had significant antihypertensive action by oral administration in spontaneously hypertensive rats (SHR).
AB - The acidic-subunit of 11S seed storage globulin of Amaranthus hypochondriacus was recently modified by the incorporation of antihypertensive biopeptides: four units of Val-Tyr dipeptides (VY) in tandem and one of Ile-Pro-Pro tripeptide (IPP), was named AMC3. The in vivo effect of AMC3 enzymatic hydrolysates (AEH) produced in Escherichia coli, was evaluated in spontaneously hypertensive rats (SHR) by once-oral administration experiments. As positive control a SHR group received a dose of captopril (50. mg/kg) (an angiotensin-converting enzyme (ACE) inhibitor used for the treatment of hypertension). Mean arterial pressure (MAP) was measured for 6. h after AEH or captopril administration. AEH administration at maximal dose (100. mg/kg) significantly reduced MAP similar to the group treated with captopril. The maximal reduction in MAP was observed after 3.5. h after AEH oral administration. The present study showed that enzymatic hydrolysates of AMC3 containing ACE inhibitory peptides (4xVY and IPP) sequences had significant antihypertensive action by oral administration in spontaneously hypertensive rats (SHR).
KW - 11S acidic-subunit
KW - Amaranth globulin
KW - Antihypertensive activity
KW - Mean arterial pressure
KW - Spontaneously hypertensive rats
UR - http://www.scopus.com/inward/record.url?scp=84880506290&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2013.06.001
DO - 10.1016/j.jff.2013.06.001
M3 - Artículo
AN - SCOPUS:84880506290
SN - 1756-4646
VL - 5
SP - 1441
EP - 1449
JO - Journal of Functional Foods
JF - Journal of Functional Foods
IS - 3
ER -