An Unexpected Case of Lagochilascariasis: Interdisciplinary Management and Use of 12S and 18S rDNA Analysis

Fernando Martinez-Hernandez, Hector Manuel Prado-Calleros, Juan Pablo Ramirez-Hinojosa, Vladimir Figueroa-Angel, Maria Teresa Lopez-Reynoso, Maria del Carmen Jimenez-Andrade, Isaias Estrada-Moscoso, Nancy Rivas, Javier Escobedo-Ortegon, Ana Flisser, Mirza Gabriela Romero-Valdovinos, Pablo Maravilla

    Research output: Contribution to journalReview article


    A Mexican 24-year-old male patient was referred to our hospital due to increased left retroauricular volume with skin fistulisation, resembling an infection by the uncommon worm Lagochilascaris minor. The patient was submitted to lateral skull base surgery. No adult worms or eggs were observed during light and scanning electron microscopy analysis, as well as by histopathologic examination of the small piece of removed tissue, only L3 stage larvae of Lagochilascaris spp. were identified. Polymerase chain reaction-sequencing assays were performed using primers for the mitochondrial 12S and the nuclear 18S rDNA gene. DNA of some L minor adults, previously identified, were used as control. The molecular analysis identified the worm as L minor. According to previous reports, lagochilascariasis is a complicated infection that requires an interdisciplinary management by different clinical specialists. This is the first time that 12S and 18S rDNA genes are reported as molecular markers for diagnosis of L minor.

    Original languageEnglish
    Pages (from-to)235-241
    Number of pages7
    JournalAmerican Journal of the Medical Sciences
    Issue number4
    StatePublished - Apr 2020



    • Lagochilascariasis
    • Lagochilascaris minor, 12S rDNA, 18S rDNA

    Cite this

    Martinez-Hernandez, F., Prado-Calleros, H. M., Ramirez-Hinojosa, J. P., Figueroa-Angel, V., Lopez-Reynoso, M. T., Jimenez-Andrade, M. D. C., Estrada-Moscoso, I., Rivas, N., Escobedo-Ortegon, J., Flisser, A., Romero-Valdovinos, M. G., & Maravilla, P. (2020). An Unexpected Case of Lagochilascariasis: Interdisciplinary Management and Use of 12S and 18S rDNA Analysis. American Journal of the Medical Sciences, 359(4), 235-241.