TY - JOUR
T1 - An Unexpected Case of Lagochilascariasis
T2 - Interdisciplinary Management and Use of 12S and 18S rDNA Analysis
AU - Martinez-Hernandez, Fernando
AU - Prado-Calleros, Hector Manuel
AU - Ramirez-Hinojosa, Juan Pablo
AU - Figueroa-Angel, Vladimir
AU - Lopez-Reynoso, Maria Teresa
AU - Jimenez-Andrade, Maria del Carmen
AU - Estrada-Moscoso, Isaias
AU - Rivas, Nancy
AU - Escobedo-Ortegon, Javier
AU - Flisser, Ana
AU - Romero-Valdovinos, Mirza Gabriela
AU - Maravilla, Pablo
N1 - Publisher Copyright:
© 2020 Southern Society for Clinical Investigation
PY - 2020/4
Y1 - 2020/4
N2 - A Mexican 24-year-old male patient was referred to our hospital due to increased left retroauricular volume with skin fistulisation, resembling an infection by the uncommon worm Lagochilascaris minor. The patient was submitted to lateral skull base surgery. No adult worms or eggs were observed during light and scanning electron microscopy analysis, as well as by histopathologic examination of the small piece of removed tissue, only L3 stage larvae of Lagochilascaris spp. were identified. Polymerase chain reaction-sequencing assays were performed using primers for the mitochondrial 12S and the nuclear 18S rDNA gene. DNA of some L minor adults, previously identified, were used as control. The molecular analysis identified the worm as L minor. According to previous reports, lagochilascariasis is a complicated infection that requires an interdisciplinary management by different clinical specialists. This is the first time that 12S and 18S rDNA genes are reported as molecular markers for diagnosis of L minor.
AB - A Mexican 24-year-old male patient was referred to our hospital due to increased left retroauricular volume with skin fistulisation, resembling an infection by the uncommon worm Lagochilascaris minor. The patient was submitted to lateral skull base surgery. No adult worms or eggs were observed during light and scanning electron microscopy analysis, as well as by histopathologic examination of the small piece of removed tissue, only L3 stage larvae of Lagochilascaris spp. were identified. Polymerase chain reaction-sequencing assays were performed using primers for the mitochondrial 12S and the nuclear 18S rDNA gene. DNA of some L minor adults, previously identified, were used as control. The molecular analysis identified the worm as L minor. According to previous reports, lagochilascariasis is a complicated infection that requires an interdisciplinary management by different clinical specialists. This is the first time that 12S and 18S rDNA genes are reported as molecular markers for diagnosis of L minor.
KW - Lagochilascariasis
KW - Lagochilascaris minor, 12S rDNA, 18S rDNA
UR - http://www.scopus.com/inward/record.url?scp=85077933701&partnerID=8YFLogxK
U2 - 10.1016/j.amjms.2020.01.001
DO - 10.1016/j.amjms.2020.01.001
M3 - Artículo de revisión
C2 - 31959368
AN - SCOPUS:85077933701
SN - 0002-9629
VL - 359
SP - 235
EP - 241
JO - American Journal of the Medical Sciences
JF - American Journal of the Medical Sciences
IS - 4
ER -