TY - JOUR
T1 - Variability in Susceptibility to Type I Interferon Response and Subgenomic RNA Accumulation Between Clinical Isolates of Dengue and Zika Virus From Oaxaca Mexico Correlate With Replication Efficiency in Human Cells and Disease Severity
AU - Castro-Jiménez, Tannya Karen
AU - Gómez-Legorreta, Laura Cristina
AU - López-Campa, Laura Alejandra
AU - Martínez-Torres, Valeria
AU - Alvarado-Silva, Marcos
AU - Posadas-Mondragón, Araceli
AU - Díaz-Lima, Nallely
AU - Angulo-Mendez, Hilda Arcelia
AU - Mejía-Domínguez, Nancy R.
AU - Vaca-Paniagua, Felipe
AU - Ávila-Moreno, Federico
AU - García-Cordero, Julio
AU - Cedillo-Barrón, Leticia
AU - Aguilar-Ruíz, Sergio Roberto
AU - Bustos-Arriaga, José
N1 - Publisher Copyright:
Copyright © 2022 Castro-Jiménez, Gómez-Legorreta, López-Campa, Martínez-Torres, Alvarado-Silva, Posadas-Mondragón, Díaz-Lima, Angulo-Mendez, Mejía-Domínguez, Vaca-Paniagua, Ávila-Moreno, García-Cordero, Cedillo-Barrón, Aguilar-Ruíz and Bustos-Arriaga.
PY - 2022/6/21
Y1 - 2022/6/21
N2 - Dengue and Zika viruses cocirculate annually in endemic areas of Mexico, causing outbreaks of different magnitude and severity every year, suggesting a continuous selection of Flavivirus variants with variable phenotypes of transmissibility and virulence. To evaluate if Flavivirus variants with different phenotypes cocirculate during outbreaks, we isolated dengue and Zika viruses from blood samples of febrile patients from Oaxaca City during the 2016 and 2019 epidemic years. We compared their replication kinetics in human cells, susceptibility to type I interferon antiviral response, and the accumulation of subgenomic RNA on infected cells. We observed correlations between type I interferon susceptibility and subgenomic RNA accumulation, with high hematocrit percentage and thrombocytopenia. Our results suggest that Flaviviruses that cocirculate in Oaxaca, Mexico, have variable sensitivity to the antiviral activity of type I interferons, and this phenotypic trait correlates with the severity of the disease.
AB - Dengue and Zika viruses cocirculate annually in endemic areas of Mexico, causing outbreaks of different magnitude and severity every year, suggesting a continuous selection of Flavivirus variants with variable phenotypes of transmissibility and virulence. To evaluate if Flavivirus variants with different phenotypes cocirculate during outbreaks, we isolated dengue and Zika viruses from blood samples of febrile patients from Oaxaca City during the 2016 and 2019 epidemic years. We compared their replication kinetics in human cells, susceptibility to type I interferon antiviral response, and the accumulation of subgenomic RNA on infected cells. We observed correlations between type I interferon susceptibility and subgenomic RNA accumulation, with high hematocrit percentage and thrombocytopenia. Our results suggest that Flaviviruses that cocirculate in Oaxaca, Mexico, have variable sensitivity to the antiviral activity of type I interferons, and this phenotypic trait correlates with the severity of the disease.
KW - Zika
KW - dengue
KW - interferons
KW - isolates
KW - sfRNA
UR - http://www.scopus.com/inward/record.url?scp=85133612112&partnerID=8YFLogxK
U2 - 10.3389/fcimb.2022.890750
DO - 10.3389/fcimb.2022.890750
M3 - Artículo
C2 - 35800385
AN - SCOPUS:85133612112
SN - 2235-2988
VL - 12
JO - Frontiers in Cellular and Infection Microbiology
JF - Frontiers in Cellular and Infection Microbiology
M1 - 890750
ER -
