TY - JOUR
T1 - Variability in behavioral phenotypes after forced swimming-induced stress in rats is associated with expression of the glucocorticoid receptor, nurr1 and IL-1β in the hippocampus
AU - Ruiz-sánchez, Elizabeth
AU - López-ramírez, Arely M.
AU - Ruiz-chow, Ángel
AU - Calvillo, Minerva
AU - Reséndiz-albor, Aldo A.
AU - Anguiano, Brenda
AU - Rojas, Patricia
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12/1
Y1 - 2021/12/1
N2 - Individual differences in coping with stress may determine either a vulnerable or resilient phenotype. Therefore, it is important to better understand the biology underlying the behavioral phenotype. We assessed whether individual behavioral phenotype to acute stress is related with the hippocampal expression of glucocorticoid receptor (GR), Nurr1, interleukin-1 beta (IL-1β) or brain-derived neurotrophic factor (BDNF). Wistar male rats were exposed to forced swimming for 15 min and sacrificed at different times. Behavioral response was analyzed, and it was compared with the gene and protein expression of GR, Nurr1, IL-1β and BDNF in the hippocampus for each time point. Behavioral phenotyping showed a group with high immobility (vulnerable) while another had low immobility (resilient). No significant differences were found in the Nurr1, IL-1β and BDNF mRNA levels between resilient and vulnerable rats at different recovery times except for Nr3c1 (gene for GR). However, exposure to stress caused significantly higher levels of GR, Nurr1 and IL-1β proteins of vulnerable compared to resilient rats. This variability of behavioral phenotypes is associated with a differential molecular response to stress that involves GR, Nurr1, and IL- 1β as mediators in coping with stress. This contributes to identifying biomarkers of susceptibility to stress.
AB - Individual differences in coping with stress may determine either a vulnerable or resilient phenotype. Therefore, it is important to better understand the biology underlying the behavioral phenotype. We assessed whether individual behavioral phenotype to acute stress is related with the hippocampal expression of glucocorticoid receptor (GR), Nurr1, interleukin-1 beta (IL-1β) or brain-derived neurotrophic factor (BDNF). Wistar male rats were exposed to forced swimming for 15 min and sacrificed at different times. Behavioral response was analyzed, and it was compared with the gene and protein expression of GR, Nurr1, IL-1β and BDNF in the hippocampus for each time point. Behavioral phenotyping showed a group with high immobility (vulnerable) while another had low immobility (resilient). No significant differences were found in the Nurr1, IL-1β and BDNF mRNA levels between resilient and vulnerable rats at different recovery times except for Nr3c1 (gene for GR). However, exposure to stress caused significantly higher levels of GR, Nurr1 and IL-1β proteins of vulnerable compared to resilient rats. This variability of behavioral phenotypes is associated with a differential molecular response to stress that involves GR, Nurr1, and IL- 1β as mediators in coping with stress. This contributes to identifying biomarkers of susceptibility to stress.
KW - Behavioral phenotype
KW - Glucocorticoid receptor
KW - IL-1β
KW - Individual differences
KW - Nurr1
KW - Resilient phenotype
KW - Stress resilience
KW - Vulnerable phenotype
UR - http://www.scopus.com/inward/record.url?scp=85119608102&partnerID=8YFLogxK
U2 - 10.3390/ijms222312700
DO - 10.3390/ijms222312700
M3 - Artículo
C2 - 34884503
AN - SCOPUS:85119608102
SN - 1661-6596
VL - 22
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 23
M1 - 12700
ER -