TY - CHAP
T1 - Using Hyper as a molecular probe to visualize hydrogen peroxide in living plant cells
T2 - An updated method
AU - Lara-Rojas, Fernando
AU - Juárez-Verdayes, M. A.
AU - Wu, Hen Ming
AU - Cheung, Alice Y.
AU - Montiel, Jesus
AU - Pascual-Morales, Edgar
AU - Ryken, Samantha E.
AU - Bezanilla, Magdalena
AU - Cardenas, Luis
N1 - Publisher Copyright:
© 2023 Elsevier Inc.
PY - 2023/1
Y1 - 2023/1
N2 - Reactive oxygen species (ROS) are highly reactive reduced oxygen molecules that play a myriad of roles in animal and plant cells. In plant cells the production of ROS results from aerobic metabolism during respiration and photosynthesis. Therefore mitochondria, chloroplasts, and peroxisomes constitute an important source of ROS. However, ROS can also be produced in response to many physiological stimuli such as pathogen attack, hormone signaling, abiotic stresses or during cell wall organization and plant morphogenesis. The study of ROS in plant cells has been limited to biochemical assays and use of fluorescent probes, however, the irreversible oxidation of the fluorescent dyes prevents the visualization of dynamic changes. We have previously reported that Hyper 1 is a biosensor for H2O2 and consists of a circularly permutated YFP (cpYFP) inserted into the regulatory domain of the Escherichia coli hydrogen peroxide (H2O2) sensor protein OxyR rendering it an H2O2-specific quantitative probe (Bilan & Belousov, 2018; Hernandez-Barrera et al., 2015). Herein we describe an updated protocol for using the improved new version of Hyper 2 and Hyper 3 as a dynamic biosensor for H2O2 in Arabidopsis with virtually unlimited potential to detect H2O2 throughout the plant and under a broad range of developmental and environmental conditions (Bilan et al., 2013).
AB - Reactive oxygen species (ROS) are highly reactive reduced oxygen molecules that play a myriad of roles in animal and plant cells. In plant cells the production of ROS results from aerobic metabolism during respiration and photosynthesis. Therefore mitochondria, chloroplasts, and peroxisomes constitute an important source of ROS. However, ROS can also be produced in response to many physiological stimuli such as pathogen attack, hormone signaling, abiotic stresses or during cell wall organization and plant morphogenesis. The study of ROS in plant cells has been limited to biochemical assays and use of fluorescent probes, however, the irreversible oxidation of the fluorescent dyes prevents the visualization of dynamic changes. We have previously reported that Hyper 1 is a biosensor for H2O2 and consists of a circularly permutated YFP (cpYFP) inserted into the regulatory domain of the Escherichia coli hydrogen peroxide (H2O2) sensor protein OxyR rendering it an H2O2-specific quantitative probe (Bilan & Belousov, 2018; Hernandez-Barrera et al., 2015). Herein we describe an updated protocol for using the improved new version of Hyper 2 and Hyper 3 as a dynamic biosensor for H2O2 in Arabidopsis with virtually unlimited potential to detect H2O2 throughout the plant and under a broad range of developmental and environmental conditions (Bilan et al., 2013).
UR - http://www.scopus.com/inward/record.url?scp=85143583759&partnerID=8YFLogxK
U2 - 10.1016/bs.mie.2022.09.012
DO - 10.1016/bs.mie.2022.09.012
M3 - Capítulo
C2 - 37087192
AN - SCOPUS:85143583759
SN - 9780443131974
T3 - Methods in Enzymology
SP - 265
EP - 289
BT - Biochemical Pathways and Environmental Responses in Plants
A2 - Jez, Joseph
PB - Academic Press Inc.
ER -