TY - JOUR
T1 - Using amplified fragment length polymorphisms (AFLP) to study genetic variability in several freshwater rotifer species
AU - Hernández-Delgado, Sanjuana
AU - Mayek-Pérez, Netzahualcoyotl
AU - Santos-Medrano, Gustavo Emilio
AU - Rico-Martínez, Roberto
PY - 2005/9
Y1 - 2005/9
N2 - We have used amplified fragment length polymorphisms (AFLP) to investigate the potential of this technique as a tool to measure genetic variability in eight species of freshwater rotifers: Brachionus calyciflorus, Lecane bulla, L. luna, L. quadridentata, Plationus patulus, Philodina acuticornis odiosa, Rotaria neptunia, and R. rotatoria. We used nine combinations of oligonucleotides. We observed a total of 806 amplified bands, 798 polymorphic and 8 monomorphic. The data were analyzed using cluster analysis with UPGMA, first within each set of oligonucleotide combination and finally using all nine combinations. Our best dendrogram clearly separated monogononts from digononts, and grouped the species of monogononts in the two genera. However, it grouped R. neptunia with P. acuticornis odiosa rather than with R. rotatoria. These results are discussed in view of recent works in the literature measuring genetic variability and discussing the phylogeny of the Rotifera.
AB - We have used amplified fragment length polymorphisms (AFLP) to investigate the potential of this technique as a tool to measure genetic variability in eight species of freshwater rotifers: Brachionus calyciflorus, Lecane bulla, L. luna, L. quadridentata, Plationus patulus, Philodina acuticornis odiosa, Rotaria neptunia, and R. rotatoria. We used nine combinations of oligonucleotides. We observed a total of 806 amplified bands, 798 polymorphic and 8 monomorphic. The data were analyzed using cluster analysis with UPGMA, first within each set of oligonucleotide combination and finally using all nine combinations. Our best dendrogram clearly separated monogononts from digononts, and grouped the species of monogononts in the two genera. However, it grouped R. neptunia with P. acuticornis odiosa rather than with R. rotatoria. These results are discussed in view of recent works in the literature measuring genetic variability and discussing the phylogeny of the Rotifera.
KW - AFLP
KW - DNA
KW - Genetic diversity
KW - Phylogeny
KW - Rotifera
UR - http://www.scopus.com/inward/record.url?scp=24144494511&partnerID=8YFLogxK
U2 - 10.1007/s10750-005-4106-5
DO - 10.1007/s10750-005-4106-5
M3 - Artículo
AN - SCOPUS:24144494511
SN - 0018-8158
VL - 546
SP - 109
EP - 115
JO - Hydrobiologia
JF - Hydrobiologia
IS - 1
ER -