TY - JOUR
T1 - Urban PM2.5 activates GAPDH and induces RBC damage in COPD patients
AU - Montoya-Estrada, Araceli
AU - Torres-Ramos, Yessica D.
AU - Flores-Pliego, Arturo
AU - Ramirez-Venegas, Alejandra
AU - Ceballos-Reyes, Guillermo M.
AU - Guzman-Grenfell, Alberto M.
AU - Hicks, Juan J.
PY - 2013/1/1
Y1 - 2013/1/1
N2 - During Chronic Obstructive Pulmonary Disease (COPD) progression, the intracellular antioxidant defence in RBCs must preserve the integrity of the plasmalemma through NADPH+ generation to obtain a sufficient number of reduced non-protein SH-groups. Here, we studied the activities of enzymes in RBCs that are related to glutathione metabolism under conditions of increasing oxidative stress, which are associated with COPD progression, by increasing cellular damage in vitro with PM2.5, a ROS generator. The study included 43 patients, who were separated according to their GOLD classification into moderate and severe groups, along with 11 healthy volunteers (HV). Blood samples were analysed for G6PD, GAPDH, GPx, and GR. The results showed significant decreases in the oxidation of the G6PD, GR and GPx proteins, resulting in decreased enzymatic activity. By contrast, an increase (p<0.05) in GAPDH was observed, suggesting a pool of ATP on the membrane. However, it is evident that RBCs are damaged during the progression of COPD, although their integrity is preserved, and they retain limited function, thus allowing patient survival without haemolysis.
AB - During Chronic Obstructive Pulmonary Disease (COPD) progression, the intracellular antioxidant defence in RBCs must preserve the integrity of the plasmalemma through NADPH+ generation to obtain a sufficient number of reduced non-protein SH-groups. Here, we studied the activities of enzymes in RBCs that are related to glutathione metabolism under conditions of increasing oxidative stress, which are associated with COPD progression, by increasing cellular damage in vitro with PM2.5, a ROS generator. The study included 43 patients, who were separated according to their GOLD classification into moderate and severe groups, along with 11 healthy volunteers (HV). Blood samples were analysed for G6PD, GAPDH, GPx, and GR. The results showed significant decreases in the oxidation of the G6PD, GR and GPx proteins, resulting in decreased enzymatic activity. By contrast, an increase (p<0.05) in GAPDH was observed, suggesting a pool of ATP on the membrane. However, it is evident that RBCs are damaged during the progression of COPD, although their integrity is preserved, and they retain limited function, thus allowing patient survival without haemolysis.
KW - Antioxidant enzyme
KW - COPD
KW - Glutathione
KW - Glycolysis
KW - Oxidative stress
KW - Particulate matter
KW - Red blood cell
UR - http://www.scopus.com/inward/record.url?scp=84883664374&partnerID=8YFLogxK
U2 - 10.2741/s396
DO - 10.2741/s396
M3 - Artículo
SN - 1945-0516
VL - 5 S
SP - 638
EP - 649
JO - Frontiers in Bioscience - Scholar
JF - Frontiers in Bioscience - Scholar
IS - 2
ER -