TY - JOUR
T1 - Unusual diversity of acquired β-lactamases in multidrug-resistant pseudomonas aeruginosa isolates in a Mexican hospital
AU - Castillo-Vera, Jane
AU - Ribas-Aparicio, Rosa María
AU - Nicolau, Carlos Juan
AU - Oliver, Antonio
AU - Osorio-Carranza, Lourdes
AU - Aparicio-Ozores, Gerardo
PY - 2012/10/1
Y1 - 2012/10/1
N2 - Aims: To investigate the presence of extended spectrum and metallo β-lactamases (MBLs) in Pseudomonas aeruginosa isolates which are resistant to imipenem and ceftazidime that were isolated in a hospital in Mexico. Results: Pulsed-field gel electrophoresis (PFGE) revealed the presence of four clonal types among the 14 isolates. All these genes were found either alone or simultaneously in the P. aeruginosa strains in the following five different arrangements: < blaGES-5 > ; < blaGES-5, blaVIM-11 > ; < blaGES-5, blaVIM-2, blaVIM-11 > ; < blaGES-5, blaOXA-2 > ; and < blaGES-5, blaVIM-2, blaVIM-11, and blaOXA-2 > . Class 1 integrons were detected and contained the cassettes blaGES-5 and blaOXA-2, but not that of blaVIM. blaVIM genes occurred only in the chromosome, while blaGES-5 was located in the chromosome and in the plasmids. Conclusions: To our knowledge, this is the first description of P. aeruginosa strains simultaneously producing the VIM-2 and VIM-11 variants, and the combination of GES-5 and MBL carbapenemases, which determines a major challenge for the clinical microbiology laboratory and a remarkable epidemiological risk for the nosocomial spread of multidrug-resistant determinants.
AB - Aims: To investigate the presence of extended spectrum and metallo β-lactamases (MBLs) in Pseudomonas aeruginosa isolates which are resistant to imipenem and ceftazidime that were isolated in a hospital in Mexico. Results: Pulsed-field gel electrophoresis (PFGE) revealed the presence of four clonal types among the 14 isolates. All these genes were found either alone or simultaneously in the P. aeruginosa strains in the following five different arrangements: < blaGES-5 > ; < blaGES-5, blaVIM-11 > ; < blaGES-5, blaVIM-2, blaVIM-11 > ; < blaGES-5, blaOXA-2 > ; and < blaGES-5, blaVIM-2, blaVIM-11, and blaOXA-2 > . Class 1 integrons were detected and contained the cassettes blaGES-5 and blaOXA-2, but not that of blaVIM. blaVIM genes occurred only in the chromosome, while blaGES-5 was located in the chromosome and in the plasmids. Conclusions: To our knowledge, this is the first description of P. aeruginosa strains simultaneously producing the VIM-2 and VIM-11 variants, and the combination of GES-5 and MBL carbapenemases, which determines a major challenge for the clinical microbiology laboratory and a remarkable epidemiological risk for the nosocomial spread of multidrug-resistant determinants.
UR - http://www.scopus.com/inward/record.url?scp=84866488131&partnerID=8YFLogxK
U2 - 10.1089/mdr.2011.0183
DO - 10.1089/mdr.2011.0183
M3 - Artículo
C2 - 22554004
SN - 1076-6294
VL - 18
SP - 471
EP - 478
JO - Microbial Drug Resistance
JF - Microbial Drug Resistance
IS - 5
ER -