TY - JOUR
T1 - Trophozoites of Entamoeba histolytica express a CD59-like molecule in human colon
AU - Ventura-Juárez, J.
AU - Campos-Rodríguez, R.
AU - Jarillo-Luna, R. A.
AU - Muñoz-Fernández, L.
AU - Escario-G-Trevijano, J. A.
AU - Pérez-Serrano, J.
AU - Quintanar, J. L.
AU - Salinas, E.
AU - Villalobos-Gómez, F. R.
N1 - Funding Information:
Acknowledgments The authors wish to thank TLQ Rosa Isela Sandoval Lozano, Morphology Department, UAA and Antonio Priego Gómez and José Antonio Pérez Hernández from the Servicio de Microscopia Electrónica, Universidad de Alcalá de Henares, Spain, for their skillful technical assistance. Thanks to CONACYT for the grant V49749-M and to UAA for the support PIBB 07-4.
PY - 2009/3
Y1 - 2009/3
N2 - In vitro studies have proved the presence of epitopes of CD59 in the surface of trophozoites of Entamoeba histolytica (E. histolytica). However, it has not been proved if CD59 molecules are expressed in the surface during the trophozoites' tissue invasion. The aim of the present study was to determine whether the complement-regulatory protein CD59 is present on trophozoites of E. histolytica in human colon. Eleven specimens of amoebic colitis were studied by immunohistochemistry and electron microscopy techniques with a monoclonal antibody against human CD59 molecule. Our results show that a CD59-like molecule is expressed in trophozoites of E. histolytica found in colonic amebic lesions. Also, a CD59-like molecule was detected by western blot analysis in whole lysate of E. histolytica as well as on the plasma membrane by immunocytochemistry. These results suggest that E. histolytica can use CD59-like protein against the lytic action of membrane attack complex.
AB - In vitro studies have proved the presence of epitopes of CD59 in the surface of trophozoites of Entamoeba histolytica (E. histolytica). However, it has not been proved if CD59 molecules are expressed in the surface during the trophozoites' tissue invasion. The aim of the present study was to determine whether the complement-regulatory protein CD59 is present on trophozoites of E. histolytica in human colon. Eleven specimens of amoebic colitis were studied by immunohistochemistry and electron microscopy techniques with a monoclonal antibody against human CD59 molecule. Our results show that a CD59-like molecule is expressed in trophozoites of E. histolytica found in colonic amebic lesions. Also, a CD59-like molecule was detected by western blot analysis in whole lysate of E. histolytica as well as on the plasma membrane by immunocytochemistry. These results suggest that E. histolytica can use CD59-like protein against the lytic action of membrane attack complex.
UR - http://www.scopus.com/inward/record.url?scp=65549111694&partnerID=8YFLogxK
U2 - 10.1007/s00436-008-1262-3
DO - 10.1007/s00436-008-1262-3
M3 - Artículo
C2 - 19018568
SN - 0932-0113
VL - 104
SP - 821
EP - 826
JO - Parasitology Research
JF - Parasitology Research
IS - 4
ER -