TY - JOUR
T1 - Triggering receptor expressed on myeloid cells-1 expression on monocytes is associated with inflammation but not with infection in acute pancreatitis
AU - Ferat-Osorio, Eduardo
AU - Wong-Baeza, Isabel
AU - Esquivel-Callejas, Noemí
AU - Figueroa-Figueroa, Silvia
AU - Duarte-Rojo, Andrés
AU - Guzmán-Valdivia-Gómez, Gilberto
AU - Rodea-Rosas, Heriberto
AU - Torres-González, Rubén
AU - Sánchez-Fernández, Patricio
AU - Arriaga-Pizano, Lourdes
AU - López-Macías, Constantino
AU - Robles-Díaz, Guillermo
AU - Isibasi, Armando
N1 - Funding Information:
This study was supported financially by Consejo Nacional de Ciencia y Tecnología (CONACyT) (grant no. SALUD-2005-01-13942 to A. Isibasi and SALUD-2004-01-132 to C. López-Macías). E. Ferat-Osorio and I. Wong-Baeza received scholarships from CONACyT and I. Wong-Baeza and N. Esquivel-Callejas from IMSS.
PY - 2009/5/14
Y1 - 2009/5/14
N2 - Introduction: Acute pancreatitis (AP) is usually a mild and self-limiting disease, but some patients develop a severe form that is associated with high mortality. In AP, local inflammation is followed first by the systemic inflammatory response syndrome and then by the compensatory anti-inflammatory response syndrome, which is defined by low human leukocyte antigen (HLA)-DR expression on monocytes, increased concentration of the anti-inflammatory cytokine IL-10, and decreased monocyte function. Our aim was to measure the expression of triggering receptor expressed on myeloid cells (TREM)-1 (a proposed marker of infection or inflammation) and HLA-DR on monocytes, and the serum concentrations of IL-6 (a proinflammatory cytokine) and IL-10 in patients with AP to determine whether these markers can identify patients at high risk of developing severe AP or infection. Methods: Fifty healthy volunteers, 18 patients with mild AP, and 11 patients with severe AP were included in this study. Samples were taken at admission and one and three days later. TREM-1 and HLA-DR expression was evaluated by flow cytometry, and soluble TREM-1, IL-6 and IL-10 concentrations were measured by ELISA. Results: TREM-1 expression was higher in patients with AP than in healthy volunteers, but there was no difference between patients with mild and severe AP. TREM-1 expression was not associated with mortality or with the presence of infection. Soluble TREM-1 concentration in serum was higher in non-survivors than in survivors. HLA-DR expression was lower and IL-6 concentration higher in patients with severe AP and in infected patients. Conclusions: Increased TREM-1 expression was associated with the presence of inflammation but not infection in AP. In patients with AP, low HLA-DR expression and high IL-6 concentration could predict severity and infection in samples taken shortly after admission.
AB - Introduction: Acute pancreatitis (AP) is usually a mild and self-limiting disease, but some patients develop a severe form that is associated with high mortality. In AP, local inflammation is followed first by the systemic inflammatory response syndrome and then by the compensatory anti-inflammatory response syndrome, which is defined by low human leukocyte antigen (HLA)-DR expression on monocytes, increased concentration of the anti-inflammatory cytokine IL-10, and decreased monocyte function. Our aim was to measure the expression of triggering receptor expressed on myeloid cells (TREM)-1 (a proposed marker of infection or inflammation) and HLA-DR on monocytes, and the serum concentrations of IL-6 (a proinflammatory cytokine) and IL-10 in patients with AP to determine whether these markers can identify patients at high risk of developing severe AP or infection. Methods: Fifty healthy volunteers, 18 patients with mild AP, and 11 patients with severe AP were included in this study. Samples were taken at admission and one and three days later. TREM-1 and HLA-DR expression was evaluated by flow cytometry, and soluble TREM-1, IL-6 and IL-10 concentrations were measured by ELISA. Results: TREM-1 expression was higher in patients with AP than in healthy volunteers, but there was no difference between patients with mild and severe AP. TREM-1 expression was not associated with mortality or with the presence of infection. Soluble TREM-1 concentration in serum was higher in non-survivors than in survivors. HLA-DR expression was lower and IL-6 concentration higher in patients with severe AP and in infected patients. Conclusions: Increased TREM-1 expression was associated with the presence of inflammation but not infection in AP. In patients with AP, low HLA-DR expression and high IL-6 concentration could predict severity and infection in samples taken shortly after admission.
UR - http://www.scopus.com/inward/record.url?scp=66749163092&partnerID=8YFLogxK
U2 - 10.1186/cc7876
DO - 10.1186/cc7876
M3 - Artículo
C2 - 19442309
SN - 1364-8535
VL - 13
JO - Critical Care
JF - Critical Care
IS - 3
M1 - R69
ER -