TY - JOUR
T1 - Trichoderma asperellum induces maize seedling growth by activating the plasma membrane H+-ATPase
AU - Ĺopez-Coria, M.
AU - Herńandez-Mendoza, J. L.
AU - Śanchez-Nieto, S.
N1 - Publisher Copyright:
© 2016 The American Phytopathological Society.
PY - 2016/10
Y1 - 2016/10
N2 - Although Trichoderma spp. have beneficial effects on numerous plants, there is not enough knowledge about the mechanism by which they improves plant growth. In this study, we evaluated the participation of plasma membrane (PM) H+-ATPase, a key enzyme involved in promoting cell growth, in the elongation induced by T. asperellum and compared it with the effect of 10 mM indol acetic acid (IAA) because IAA promotes elongation and PM H+-ATPase activation. Two seed treatments were tested: biopriming and noncontact. In neither were the tissues colonized by T. asperellum; however, the seedlings were longer than the control seedlings, which also accumulated IAA and increased root acidification. An auxin transport inhibitor (2,3,5 triiodobenzoic acid) reduced the plant elongation induced by Trichoderma spp. T. asperellum seed treatment increased the PM H+-ATPase activity in plant roots and shoots. Additionally, the T. asperellum extracellular extract (TE) activated the PM H+-ATPase activity of microsomal fractions of control plants, although it contained 0.3mMIAA. Furthermore, themechanismof activation of PMH+-ATPase was different for IAA and TE; in the latter, the activation depends on the phosphorylation state of the enzyme, suggesting that, in addition to IAA, T. asperellum excretes othermolecules that stimulate PM H+-ATPase to induce plant growth.
AB - Although Trichoderma spp. have beneficial effects on numerous plants, there is not enough knowledge about the mechanism by which they improves plant growth. In this study, we evaluated the participation of plasma membrane (PM) H+-ATPase, a key enzyme involved in promoting cell growth, in the elongation induced by T. asperellum and compared it with the effect of 10 mM indol acetic acid (IAA) because IAA promotes elongation and PM H+-ATPase activation. Two seed treatments were tested: biopriming and noncontact. In neither were the tissues colonized by T. asperellum; however, the seedlings were longer than the control seedlings, which also accumulated IAA and increased root acidification. An auxin transport inhibitor (2,3,5 triiodobenzoic acid) reduced the plant elongation induced by Trichoderma spp. T. asperellum seed treatment increased the PM H+-ATPase activity in plant roots and shoots. Additionally, the T. asperellum extracellular extract (TE) activated the PM H+-ATPase activity of microsomal fractions of control plants, although it contained 0.3mMIAA. Furthermore, themechanismof activation of PMH+-ATPase was different for IAA and TE; in the latter, the activation depends on the phosphorylation state of the enzyme, suggesting that, in addition to IAA, T. asperellum excretes othermolecules that stimulate PM H+-ATPase to induce plant growth.
UR - http://www.scopus.com/inward/record.url?scp=84994577479&partnerID=8YFLogxK
U2 - 10.1094/MPMI-07-16-0138-R
DO - 10.1094/MPMI-07-16-0138-R
M3 - Artículo
C2 - 27643387
SN - 0894-0282
VL - 29
SP - 797
EP - 806
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
IS - 10
ER -