TY - JOUR
T1 - Transient receptor potential (TRPC) channels in human sperm
T2 - Expression, cellular localization and involvement in the regulation of flagellar motility
AU - Castellano, Laura E.
AU - Treviño, Claudia L.
AU - Rodríguez, Delany
AU - Serrano, Carmen J.
AU - Pacheco, Judith
AU - Tsutsumi, Víctor
AU - Felix, Ricardo
AU - Darszon, Alberto
N1 - Funding Information:
This work was supported by grants from DGAPA (UNAM) to A.D. and C.L.T.; from CONACyT to A.D. and to R.F. as well as from TWAS to R.F. and C.L.T. We thank X. Alvarado, A. Sandoval, E. Bustos, M. Olvera and A. Vega for expert technical assistance. We thank Laboratorio Clı́nico y de Biogenética Eugenio Sue for allowing us to use the CASA system.
PY - 2003/4/24
Y1 - 2003/4/24
N2 - Capacitative Ca2+ entry is a process whereby the activation of Ca2+ influx through the plasma membrane is triggered by depletion of intracellular Ca2+ stores. Some transient receptor potential (TRPC) proteins have been proposed as candidates for capacitative Ca2+ channels. Recent evidence indicates that capacitative Ca2+ entry participates in the sperm acrosome reaction (AR), an exocytotic process necessary for fertilization. In addition, several TRPCs have been detected heterogeneously distributed in mouse sperm, suggesting that they may participate in other functions such as motility. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, RNA messengers for TRPC1, 3, 6 and 7 were found in human spermatogenic cells. Confocal indirect immunofluorescence revealed the presence of TRPC1, 3, 4 and 6 differentially localized in the human sperm, and immunogold transmission electron microscopy indicated that TRPC epitopes are mostly associated to the surface of the cells. Because all of them were detected in the flagellum, TRPC channel antagonists were tested in sperm motility using a computer-assisted assay. Our results provide what is to our knowledge the first evidence that these channels may influence human sperm motility.
AB - Capacitative Ca2+ entry is a process whereby the activation of Ca2+ influx through the plasma membrane is triggered by depletion of intracellular Ca2+ stores. Some transient receptor potential (TRPC) proteins have been proposed as candidates for capacitative Ca2+ channels. Recent evidence indicates that capacitative Ca2+ entry participates in the sperm acrosome reaction (AR), an exocytotic process necessary for fertilization. In addition, several TRPCs have been detected heterogeneously distributed in mouse sperm, suggesting that they may participate in other functions such as motility. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, RNA messengers for TRPC1, 3, 6 and 7 were found in human spermatogenic cells. Confocal indirect immunofluorescence revealed the presence of TRPC1, 3, 4 and 6 differentially localized in the human sperm, and immunogold transmission electron microscopy indicated that TRPC epitopes are mostly associated to the surface of the cells. Because all of them were detected in the flagellum, TRPC channel antagonists were tested in sperm motility using a computer-assisted assay. Our results provide what is to our knowledge the first evidence that these channels may influence human sperm motility.
KW - Ca channel
KW - Capacitative Ca entry
KW - Sperm motility
KW - Store operated channel
KW - TRPC
KW - Transient receptor potential
UR - http://www.scopus.com/inward/record.url?scp=0037464465&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(03)00305-3
DO - 10.1016/S0014-5793(03)00305-3
M3 - Artículo
SN - 0014-5793
VL - 541
SP - 69
EP - 74
JO - FEBS Letters
JF - FEBS Letters
IS - 1-3
ER -