TY - JOUR
T1 - Three-Dimensional 3D Culture Models in Gynecological and Breast Cancer Research
AU - Salinas-Vera, Yarely M.
AU - Valdés, Jesús
AU - Pérez-Navarro, Yussel
AU - Mandujano-Lazaro, Gilberto
AU - Marchat, Laurence A.
AU - Ramos-Payán, Rosalio
AU - Nuñez-Olvera, Stephanie I.
AU - Pérez-Plascencia, Carlos
AU - López-Camarillo, César
N1 - Publisher Copyright:
Copyright © 2022 Salinas-Vera, Valdés, Pérez-Navarro, Mandujano-Lazaro, Marchat, Ramos-Payán, Nuñez-Olvera, Pérez-Plascencia and López-Camarillo.
PY - 2022/5/26
Y1 - 2022/5/26
N2 - Traditional two-dimensional (2D) monolayer cell cultures have long been the gold standard for cancer biology research. However, their ability to accurately reflect the molecular mechanisms of tumors occurring in vivo is limited. Recent development of three-dimensional (3D) cell culture models facilitate the possibility to better recapitulate several of the biological and molecular characteristics of tumors in vivo, such as cancer cells heterogeneity, cell-extracellular matrix interactions, development of a hypoxic microenvironment, signaling pathway activities depending on contacts with extracellular matrix, differential growth kinetics, more accurate drugs response, and specific gene expression and epigenetic patterns. In this review, we discuss the utilization of different types of 3D culture models including spheroids, organotypic models and patient-derived organoids in gynecologic cancers research, as well as its potential applications in oncological research mainly for screening drugs with major physiological and clinical relevance. Moreover, microRNAs regulation of cancer hallmarks in 3D cell cultures from different types of cancers is discussed.
AB - Traditional two-dimensional (2D) monolayer cell cultures have long been the gold standard for cancer biology research. However, their ability to accurately reflect the molecular mechanisms of tumors occurring in vivo is limited. Recent development of three-dimensional (3D) cell culture models facilitate the possibility to better recapitulate several of the biological and molecular characteristics of tumors in vivo, such as cancer cells heterogeneity, cell-extracellular matrix interactions, development of a hypoxic microenvironment, signaling pathway activities depending on contacts with extracellular matrix, differential growth kinetics, more accurate drugs response, and specific gene expression and epigenetic patterns. In this review, we discuss the utilization of different types of 3D culture models including spheroids, organotypic models and patient-derived organoids in gynecologic cancers research, as well as its potential applications in oncological research mainly for screening drugs with major physiological and clinical relevance. Moreover, microRNAs regulation of cancer hallmarks in 3D cell cultures from different types of cancers is discussed.
KW - 3D cultures
KW - breast cancer
KW - gynecological cancers
KW - microRNAs
KW - therapy response
UR - http://www.scopus.com/inward/record.url?scp=85132272712&partnerID=8YFLogxK
U2 - 10.3389/fonc.2022.826113
DO - 10.3389/fonc.2022.826113
M3 - Artículo de revisión
C2 - 35692756
AN - SCOPUS:85132272712
SN - 2234-943X
VL - 12
JO - Frontiers in Oncology
JF - Frontiers in Oncology
M1 - 826113
ER -