TY - JOUR
T1 - The sarcoglycan-sarcospan complex localization in mouse retina is independent from dystrophins
AU - Fort, Patrice
AU - Estrada, Francisco Javier
AU - Bordais, Agnès
AU - Mornet, Dominique
AU - Sahel, José Alain
AU - Picaud, Serge
AU - Vargas, Haydeé Rosas
AU - Coral-Vázquez, Ramón M.
AU - Rendon, Alvaro
N1 - Funding Information:
This work was supported by Institut National de la Santé Et de la Recherche Médicale (INSERM) and by grants from the Association Française contre les Myopathies (AFM) to A.R. and D.M.; P.F. received financial support from Fédération des Aveugles et handicapés visuels de France (FAF). P.F. and F.J.E from the AFM. A.B. received financial support from Retina France.
PY - 2005/9
Y1 - 2005/9
N2 - The sarcoglycan-sarcospan (SG-SSPN) complex is part of the dystrophin-glycoprotein complex that has been extensively characterized in muscle. To establish the framework for functional studies of sarcoglycans in retina here, we quantified sarcoglycans mRNA levels with real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and performed immunohistochemistry to determine their cellular and subcellular distribution. We showed that the β-, δ-, γ-, ε-sarcoglycans and sarcospan are expressed in mouse retina. They are localized predominantly in the outer and the inner limiting membranes, probably in the Müller cells and also in the ganglion cells axons where the expression of dystrophins have never been reported. We also investigated the status of the sarcoglycans in the retina of mdx3cv mutant mice for all Duchene Muscular Dystrophy (DMD) gene products. The absence of dystrophin did not produce any change in the sarcoglycan-sarcospan components expression and distribution.
AB - The sarcoglycan-sarcospan (SG-SSPN) complex is part of the dystrophin-glycoprotein complex that has been extensively characterized in muscle. To establish the framework for functional studies of sarcoglycans in retina here, we quantified sarcoglycans mRNA levels with real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and performed immunohistochemistry to determine their cellular and subcellular distribution. We showed that the β-, δ-, γ-, ε-sarcoglycans and sarcospan are expressed in mouse retina. They are localized predominantly in the outer and the inner limiting membranes, probably in the Müller cells and also in the ganglion cells axons where the expression of dystrophins have never been reported. We also investigated the status of the sarcoglycans in the retina of mdx3cv mutant mice for all Duchene Muscular Dystrophy (DMD) gene products. The absence of dystrophin did not produce any change in the sarcoglycan-sarcospan components expression and distribution.
KW - Dystrophins
KW - Mouse
KW - Retina
KW - Sarcoglycans
KW - Sarcospan
KW - mdx
UR - http://www.scopus.com/inward/record.url?scp=23044501809&partnerID=8YFLogxK
U2 - 10.1016/j.neures.2005.05.007
DO - 10.1016/j.neures.2005.05.007
M3 - Artículo
SN - 0168-0102
VL - 53
SP - 25
EP - 33
JO - Neuroscience Research
JF - Neuroscience Research
IS - 1
ER -