TY - JOUR
T1 - The PGRS domain of Mycobacterium tuberculosis. PE_PGRS Rv1759c antigen is an efficient subunit vaccine to prevent reactivation in a murine model of chronic tuberculosis
AU - Campuzano, Jaime
AU - Aguilar, Diana
AU - Arriaga, Kutzy
AU - León, Juan Carlos
AU - Salas-Rangel, Laura Patricia
AU - González-y-Merchand, Jorge
AU - Hernández-Pando, Rogelio
AU - Espitia, Clara
N1 - Funding Information:
We are grateful to Cristina Parada for technical assistance. This work was supported by grants from Conacyt (G36923-M, 33580-M), DGPA (IN221599) Universidad Nacional Autonoma de México and the European Community (INCO DC ICA4-CT-2002-10063).
PY - 2007/5/4
Y1 - 2007/5/4
N2 - A third of the world population is latently infected with Mycobacterium tuberculosis and many cases of active tuberculosis arise from latent bacilli reactivation. Thus, it is important to design new vaccines to prevent reactivation. Using an experimental model of chronic tuberculosis in B6D2F1 mice, we observed constant expression of Rv1759c antigen, a member of the PE_PGRS gene family, on the cell wall of phagocytosed mycobacteria by activated macrophages located in lung granulomas. This antigen induced production of IFN-γ after stimulation of cell suspensions from mediastinal lymph nodes. It was notorius that chronic infected mice immunized with this antigen and treated with corticosterone to induce reactivation showed not change in colony forming units (CFU), compared with the significant bacilli increase in non-vaccinated mice treated with corticosterone. These results suggest that this antigen could play an important role in the immune response that maintains latent infection, and could therefore, be a good candidate as a new subunit vaccine to prevent disease reactivation.
AB - A third of the world population is latently infected with Mycobacterium tuberculosis and many cases of active tuberculosis arise from latent bacilli reactivation. Thus, it is important to design new vaccines to prevent reactivation. Using an experimental model of chronic tuberculosis in B6D2F1 mice, we observed constant expression of Rv1759c antigen, a member of the PE_PGRS gene family, on the cell wall of phagocytosed mycobacteria by activated macrophages located in lung granulomas. This antigen induced production of IFN-γ after stimulation of cell suspensions from mediastinal lymph nodes. It was notorius that chronic infected mice immunized with this antigen and treated with corticosterone to induce reactivation showed not change in colony forming units (CFU), compared with the significant bacilli increase in non-vaccinated mice treated with corticosterone. These results suggest that this antigen could play an important role in the immune response that maintains latent infection, and could therefore, be a good candidate as a new subunit vaccine to prevent disease reactivation.
KW - Chronic tuberculosis
KW - Immunotherapy
KW - Rv1759c protein
UR - http://www.scopus.com/inward/record.url?scp=34147108365&partnerID=8YFLogxK
U2 - 10.1016/j.vaccine.2006.12.042
DO - 10.1016/j.vaccine.2006.12.042
M3 - Artículo
SN - 0264-410X
VL - 25
SP - 3722
EP - 3729
JO - Vaccine
JF - Vaccine
IS - 18
ER -