TY - JOUR
T1 - The Peptide AmPep1 Derived from Amaranth Recognizes the Replication Hairpin of TYLCV Disturbing Its Replication Process in Host Plants
AU - Mendoza-Figueroa, José S.
AU - Badillo-Ramírez, Isidro
AU - Kvarnheden, Anders
AU - Rosas-Ramírez, Daniel G.
AU - Rodríguez-Negrete, Edgar A.
AU - Méndez-Lozano, Jesús
AU - Saniger, José M.
AU - Soriano-García, Manuel
N1 - Publisher Copyright:
Copyright © 2019 American Chemical Society.
PY - 2019/4/22
Y1 - 2019/4/22
N2 - Antiviral compounds targeting viral replicative processes have been studied as an alternative for the control of begomoviruses. Previously, we have reported that the peptide AmPep1 has strong affinity binding to the replication origin sequence of tomato yellow leaf curl virus (TYLCV). In this study, we describe the mechanism of action of this peptide as a novel alternative for control of plant-infecting DNA viruses. When AmPep1 was applied exogenously to tomato and Nicotiana benthamiana plants infected with TYLCV, a decrease in the synthesis of the two viral DNA strands (CS and VS) was observed, with a consequent delay in the development of disease progress in treated plants. The chemical mechanism of action of AmPep1 was deduced using Raman spectroscopy and molecular modeling showing the formation of chemical interactions such as H bonds and electrostatic interactions and the formation of π-πinteractions between both biomolecules contributing to tampering with the viral replication.
AB - Antiviral compounds targeting viral replicative processes have been studied as an alternative for the control of begomoviruses. Previously, we have reported that the peptide AmPep1 has strong affinity binding to the replication origin sequence of tomato yellow leaf curl virus (TYLCV). In this study, we describe the mechanism of action of this peptide as a novel alternative for control of plant-infecting DNA viruses. When AmPep1 was applied exogenously to tomato and Nicotiana benthamiana plants infected with TYLCV, a decrease in the synthesis of the two viral DNA strands (CS and VS) was observed, with a consequent delay in the development of disease progress in treated plants. The chemical mechanism of action of AmPep1 was deduced using Raman spectroscopy and molecular modeling showing the formation of chemical interactions such as H bonds and electrostatic interactions and the formation of π-πinteractions between both biomolecules contributing to tampering with the viral replication.
KW - Raman spectroscopy
KW - TYLCV
KW - antiviral
KW - peptide
KW - plant virus
UR - http://www.scopus.com/inward/record.url?scp=85071425082&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.9b02526
DO - 10.1021/acs.jafc.9b02526
M3 - Artículo
C2 - 31369258
AN - SCOPUS:85071425082
SN - 0021-8561
VL - 67
SP - 9241
EP - 9253
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 33
ER -